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Histopathological examination and transcriptome analyses to assess the acute toxic effects of arsenite exposure on rare minnows (Gobiocypris rarus)

文献类型： 外文期刊

作者： Shi, Lixia 1 ; Hu, Xudong 1 ; Wang, Nenghan 1 ; Liang, Huifang 5 ; Wu, Chenxi 1 ; Cao, Hong 1 ;

作者机构： 1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat Chinese Acad, Inst Hydrobiol, Wuhan 430072, Peoples R China

2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

3.CAFS, Key Lab Freshwater Biodivers Conservat, Yangtze River Fisheries Res Inst, Minist Agr & Rural Affairs, Wuhan 430223, Peoples R China

4.Huizhou Univ, Sch Life Sci, Huizhou 516007, Peoples R China

5.Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Hepat Surg Ctr, Wuhan 430030, Peoples R China

关键词： Acute arsenic toxicity; Rare minnow; Histopathology; Mitochondria; Transcriptome

期刊名称：ECOTOXICOLOGY （ 影响因子：2.823； 五年影响因子：2.958 ）

ISSN： 0963-9292

年卷期： 2020 年 29 卷 5 期

页码：

收录情况： SCI

摘要： Arsenic is ubiquitously present in the aquatic environment. We investigated the acute toxic effects of arsenite [As(III)] exposure on rare minnows (Gobiocypris rarus) in vivo. The 96-h LC50 value for exposure to As(III) was 13.73 mg/L. As(III) bioaccumulation in different tissues was measured using inductively-coupled plasma mass spectrometry, and the extent of As(III) accumulation was, from greatest to least, liver > intestine > gills > muscle > kidney > testis > brain. Histological examination revealed that in As(III)-treated fish, numerous cellular and tissue alterations were present in the gill, liver, and intestine tissues. Moreover, transmission electron microscopy showed ultrastructural alterations in hepatocytes. We also performed transcriptome analyses to investigate As(III)-induced toxicity response in the liver of As(III)-treated fish; various oxidative-related genes were differentially expressed, and their expression levels were further validated using qPCR. This study is one of the many steps we aim to take on the way to promote the rare minnow to an international standard laboratory animal.

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