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Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals

文献类型： 外文期刊

作者： Cao, Hong 1 ; Zhou, Li 1 ; Zhang, Yan-Zhen 2 ; Wei, Qi-Wei 2 ; Chen, Xi-Hua 2 ; Gui, Jian-Fang 1 ;

作者机构： 1.Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Agr Minist China, Key Lab freshwater Fish Germplasm Resources & Bio, Jinzhou 434000, Peoples R China

关键词： Gonadotropin;Acipenser sinensis;Pituitary;AsGTH-alpha I;AsFSH beta;AsLH beta

期刊名称：MOLECULAR AND CELLULAR ENDOCRINOLOGY （ 影响因子：4.102； 五年影响因子：4.226 ）

ISSN： 0303-7207

年卷期： 2009 年 303 卷 1-2 期

页码：

收录情况： SCI

摘要： Chinese sturgeon (Acipenser sinensis) is a rare and endangered species, and also an important resource for the sturgeon aquaculture industry. To understand molecular characterization of Chinese sturgeon gonadotropins (GTHs), we cloned the full-length cDNAs of gonadotropin subunits common alpha (GTH-alpha), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from a pituitary cDNA library of mature female. Two subtypes of GTH-alpha were identified. The nucleotide sequences of A. sinensis common alpha I (AsGTH-alpha I), common alpha II (AsGTH-alpha II), FSH beta (AsFSH beta) and LH beta (AsLH beta) subunit cDNAs are 345, 363, 387 and 414 bp in length, and encode mature peptides of 115, 121, 129 and 138 aa, respectively. Then, three polyclonal antibodies were prepared from the in vitro expressed AsGTH-alpha I, AsFSH beta and AsLH beta mature proteins, respectively. Significant expression differences were revealed between immature and mature sturgeon pituitaries. Western blot detection and immunofluoresence localization revealed the existence of three-gonadotropin subunits (AsGTH-alpha, AsFSH beta and AsLH beta) in mature sturgeon pituitaries, but only AsFSH beta was detected in immature individual pituitaries during early stages in the sturgeon life, and obvious difference was observed between males and females. In males, AsFSH beta was expressed in 4-year-old individuals, whereas in females, AsFSH beta was just expressed in 5-year-old individuals. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

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