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Regioselective sulfation of beta-glucan from Ganoderma lucidum and structure-anticoagulant activity relationship of sulfated derivatives

文献类型： 外文期刊

作者： Zhang, Zhong 1 ; Tang, Qingjiu 1 ; Wu, Di 1 ; Zhou, Shuai 1 ; Yang, Yan 1 ; Feng, Na 1 ; Tang, Chuanhong 1 ; Wang, Jinyan 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Key Lab Edible Fungi Resources & Utilizat South, Inst Edible Fungi,Minist Agr, Shanghai 201403, Peoples R China

关键词： Sulfated beta-glucan; Regioselective synthesis; Structure-activity relationship

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2020 年 155 卷

页码：

收录情况： SCI

摘要： In the present study, regioselective sulfation of beta-glucan (GLP) from Ganoderma lucidum were firstly established by using 4,4'-dimethoxytrityl chloride and hexamethyldisilazane as protecting precursor. 2,4,6-O-sulfated, 6-O-sulfated and 2,4-O-sulfated GLP derivatives were prepared and the molecular weights (M-w) of derivatives were determined to range from 0.94 x 10(4) to 6.27 x 10(4) g/mol, while the degrees of sulfation (DS) were calculated to vary from 0.83 to 1.74. The regioselective sulfation of GLP was confirmed by FT-IR, C-13 NMR spectroscopy and methylation analysis. Results indicated that the sulfated substitution sites were predominantly at C-6 in 6-O-sulfated GLP (S(6-O)GLP) and C-4 in 2,4-O-sulfated GLP (S(2,4-O)GLP), respectively. Clotting assays (APTT, PT and TT) in vitro showed that sulfate groups were essential for anticoagulant activity and S(6-O)GLP exhibited much higher than others. Meanwhile, sulfated GLP with higher DS and M-w showed stronger anticoagulant activity in the case of the same condition. (C) 2020 Elsevier B.V. All rights reserved.

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