文献类型： 外文期刊
作者： Zhao, Dongmin 2 ; Zhang, Lijiao 2 ; Han, Kaikai 2 ; Liu, Qingtao 2 ; Yang, Jing 2 ; Huang, Xinmei 2 ; Liu, Yuzhuo 2 ; Li, 1 ;
作者机构： 1.Shandong Agr Univ, Coll Anim Sci & Technol, Tai An, Shandong, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Jiangsu, Peoples R China
3.Minist Agr, Key Lab Vet Biol Engn & Technol, Nanjing, Jiangsu, Peoples R China
4.Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An, Shandong, Peoples R China
5.Shandong Prov Engn Technol Res Ctr Anim Dis Contr, Tai An, Shandong, Peoples R China
关键词： Tembusu virus; Envelope protein; Inhibitory peptides; Antiviral
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）
ISSN： 0378-1135
年卷期： 2020 年 245 卷 0 期
页码：
收录情况： SCI
摘要： The outbreak and spread of Tembusu virus (TMUV) has caused very large losses in the waterfowl-breeding industry since 2010. The viral envelope (E) protein, the principal surface protein of viral particles, plays a vital role in viral entry and fusion. In this study, two peptides derived from domain II (DII) and the stem of the TMUV envelope protein, TP1 and TP2, respectively, were tested for their antiviral activity. TP1 and TP2 inhibited TMUV infection in BHK-21 cells, and their 50% inhibitory concentrations (IC50) were 14.19 mg/L and 7.64 mg/L, respectively. Viral inhibition assays in different cell lines of avian origin showed that the inhibitory effects of TP1 and TP2 are not cell type dependent. Moreover, TP2 also exhibited inhibitory activity against Japanese encephalitis virus (JEV) infection. The two peptides inhibited antibody-mediated TMUV infection of duck peripheral blood lymphocytes. Co-immunoprecipitation assays and indirect enzyme-linked immunosorbent assays (ELISAs) indicated that both peptides interact with the surface of the TMUV virion. RNase digestion assays confirmed the release of viral RNA following incubation with TP1, while incubation with TP1 or TP2 interfered with the binding between TMUV and cells. Taken together, these results show that TP1 and TP2 may be developed into antiviral treatments against TMUV infection.
- 相关文献
作者其他论文 更多>>
-
An efficient measure for the isolation of chenodeoxycholic acid from chicken biles using enzyme-assisted extraction and macroporous resins refining
作者：Yang, Biao;Lu, Fangyun;Li, Pengpeng;Ma, Jingjing;Yang, Jing;Zhang, Xinxiao;Cheng, Mei;Yu, Wenjing;Chai, Yao;Zou, Ye;Xu, Weimin;Wang, Daoying;Yang, Biao;Li, Pengpeng;Ma, Jingjing;Yang, Jing;Zhang, Xinxiao;Cheng, Mei;Chai, Yao;Zou, Ye;Xu, Weimin;Wang, Daoying;Lu, Fangyun;Yu, Wenjing
关键词：Bile salt hydrolase; Chenodeoxycholic acid; Macroporous resin; Adsorption; Purification
-
Effect of ultrasound combined with plasma protein treatment on the structure, physicochemical and rheological properties of myofibrillar protein
作者：Zou, Ye;Wang, Lingjuan;Wang, Xiaowen;Lan, Yibo;Ma, Jingjing;Yang, Jing;Xu, Weimin;Shen, Qi;Wang, Daoying;Zou, Ye;Wang, Lingjuan;Wang, Xiaowen;Xu, Weimin;Shen, Qi;Wang, Daoying
关键词：Ultrasound treatment; Plasma protein; Myofibrillar protein; Gumminess; Rheological property
-
Evaluation of Antiviral Activity of Lactoferrin in Bovine Milk Toward Fondaparinux Using Ordered Porous Layer Interferometry
作者：Zhang, Yu;Liu, Liming;Wan, Yizhen;Wang, Tianze;Zheng, Xiaoling;Zhang, Bo;Qian, Weiping;Zhao, Dongmin;Wang, Huili;Qian, Weiping
关键词：
-
Effect of low-frequency ultrasound pretreatment on taste substances in chicken liver by fermentation
作者：Wang, Xiaowen;Lan, Yibo;Yang, Biao;Yang, Jing;Ma, Jingjing;Cheng, Mei;Xia, Xiudong;Xu, Weimin;Wang, Daoying;Zou, Ye;Wang, Xiaowen;Xia, Xiudong;Xu, Weimin;Wang, Daoying;Zou, Ye;Wang, Xiaowen;Lan, Yibo;Yang, Biao;Yang, Jing;Ma, Jingjing;Cheng, Mei;Xia, Xiudong;Xu, Weimin;Wang, Daoying;Zou, Ye
关键词：Chicken liver; Fermentation; Ultrasound pretreatment; Taste; Acid soluble protein
-
Transcriptomic and metabolomic analysis reveals the effect of hydrogen sulfide on delaying fruit ripening and color transition of white fleshed strawberry (Fragaria ananassa)
作者：Sun, Miao;Qiao, Xuanhuan;Yang, Tao;Zhao, Peng;Zhao, Junhao;Luo, Jiaming;Zhang, Jialun;Xu, Meng;Yao, Li;Wen, Yue;Zhu, Zhipeng;Cai, Weijian;Sun, Miao;Yao, Li;Xiong, Aisheng
关键词：Strawberry; Fruit maturation; Hydrogen sulfide; Metabolome; RNA-Seq; Regulatory network
-
Identification of B-Cell Epitopes Located on the Surface of the S1 Protein of Infectious Bronchitis Virus M41 Strains
作者：Gao, Zichen;Hu, Jianing;Cai, Yiqin;Liu, Ye;Yin, Guihu;Guo, Xinyu;Wang, Ruiying;Zhong, Meng;Feng, Xiuli;Gao, Zichen;Hu, Jianing;Cai, Yiqin;Liu, Ye;Yin, Guihu;Guo, Xinyu;Wang, Ruiying;Zhong, Meng;Feng, Xiuli;Liu, Qingtao
关键词：avian infectious bronchitis virus; monoclonal antibody; S1 antigenic determinants; B-cell epitope; peptide scanning; overlapping fragments; prokaryotic expression
-
An mRNA vaccine provides effective protection against Duck Tembusu Virus infection
作者：Xu, Shi;Fei, Caiyi;Liu, Guilai;Xu, Mengwei;Liu, Rui;Li, Jing;Wang, Aili;He, Qing;Han, Tiyun;Zhang, Lijiao;Liu, Qingtao;Zhang, Lijiao;Liu, Qingtao;Zhang, Lijiao;Liu, Qingtao;Zhang, Lijiao;Liu, Qingtao;Cai, Ke
关键词：
