Colonization of Beauveria bassiana 08F04 in root-zone soil and its biocontrol of cereal cyst nematode (Heterodera filipjevi)
文献类型: 外文期刊
作者: Zhang, Jie 1 ; Fu, Bo 2 ; Lin, Qitong 3 ; Riley, Ian T. 4 ; Ding, Shengli 3 ; Chen, Linlin 3 ; Cui, Jiangkuan 3 ; Yang, L 1 ;
作者机构: 1.Henan Acad Agr Sci, Inst Plant Protect Res, Zhengzhou, Peoples R China
2.Henan Agr Univ, Coll Tobacco Sci, Zhengzhou, Peoples R China
3.Henan Agr Univ, Coll Plant Protect, Zhengzhou, Peoples R China
4.Nigde Omer Halisdemir Univ, Fac Agr Sci & Technol, Dept Plant Prod & Technol, Nigde, Turkey
期刊名称:PLOS ONE ( 2020影响因子:3.24; 五年影响因子:3.788 )
ISSN: 1932-6203
年卷期: 2020 年 15 卷 5 期
收录情况: SCI
摘要: Cereal cyst nematodes cause serious yield losses of wheat in Hunaghuai winter wheat growing region in China. Beauveria bassiana 08F04 isolated from the surface of cysts is a promising biological control agent for cereal cyst nematodes. As the colonization capacity is a crucial criteria to assess biocontrol effectiveness for a microbial agent candidate, we aimed to label B. bassiana 08F04 for efficient monitoring of colonization in the soil. The binary pCAM-gfp plasmid containing sgfp and hph was integrated into B. bassiana 08F04 using the Agrobacterium tumefaciens-mediated transformation. The transformation caused a significant change in mycelial and conidial yields, and in extracellular chitinase activity in some transformants. The cultural filtrates of some transformants also decreased acetylcholinesterase activity and the survival of Heterodera filipjevi second-stage juveniles relative to the wild-type strain. One transformant (G10) had a growth rate and biocontrol efficacy similar to the wild-type strain, so it was used for a pilot study of B. bassiana colonization conducted over 13 weeks. Real-time PCR results and CFU counts revealed that the population of G10 increased quickly over the first 3 weeks, then decreased slowly over the following 4 weeks before stabilizing. In addition, the application of wild-type B. bassiana 08F04 and transformant G10 significantly reduced the number of H. filipjevi females in roots by 64.4% and 60.2%, respectively. The results of this study have practical applications for ecological, biological and functional studies of B. bassiana 08F04 and for bionematicide registration.
- 相关文献
作者其他论文 更多>>
-
Automatic grading evaluation of winter wheat lodging based on deep learning
作者:Zang, Hecang;Su, Xinqi;Li, Guoqiang;Zhang, Jie;Zheng, Guoqing;Zang, Hecang;Li, Guoqiang;Zhang, Jie;Zheng, Guoqing;Su, Xinqi;Shen, Hualei;Wang, Yanjing;Hu, Weiguo
关键词:UAV image; winter wheat; deep learning; lodging degree; lodging area
-
Assessing Changes in Climatic Suitability for Sesame Cultivation in China (1978-2019) Based on Fuzzy Mathematics
作者:Wang, Xue;Huang, Ming;Li, Youjun;Wang, Xue;Zhang, Jiantao;Zhang, Jie;Zang, Hecang;Hu, Feng;Li, Guoqiang;Zhang, Jiantao;Zhang, Jie;Zang, Hecang;Hu, Feng;Li, Guoqiang;Gao, Tongmei
关键词:sesame (Sesamum indicum L.); climate indicators; climatic suitability; climate change
-
Genomics Analysis Reveals the Potential Biocontrol Mechanism of Pseudomonas aeruginosa QY43 against Fusarium pseudograminearum
作者:Meng, Jiaxing;Zan, Feifei;Liu, Zheran;Zhang, Yuan;Qin, Cancan;Hao, Lingjun;Wang, Zhifang;Wang, Limin;Li, Honglian;Li, Haiyang;Ding, Shengli;Liu, Dongmei;Liang, Shen;Li, Honglian;Ding, Shengli
关键词:Fusarium crown rot; Fusarium pseudograminearum; biocontrol bacteria; Pseudomonas aeruginosa; genomics; biocontrol factors
-
A novel metal-organic framework based electrochemical immunosensor for the rapid detection of Salmonella typhimurium detection in milk
作者:Zhan, Ke;Chen, Linlin;Yu, Qiuying;Zhao, Zheng;Li, Junwei;Ren, Hongtao;Li, Shanshan;Wang, Na;Zhan, Ke;Chen, Linlin;Li, Shanshan;Yu, Qiuying;Zhao, Zheng;Li, Junwei;Ren, Hongtao;Wang, Na;Zhang, Gaiping;Zhan, Ke;Chen, Linlin;Li, Shanshan;Yu, Qiuying;Zhao, Zheng;Li, Junwei;Ren, Hongtao;Wang, Na;Zhang, Gaiping;Zhang, Gaiping;Zhan, Ke;Chen, Linlin;Li, Shanshan;Zhao, Zheng;Li, Junwei;Xing, Yunrui;Ren, Hongtao;Wang, Na;Zhang, Gaiping;Zhan, Ke;Chen, Linlin;Li, Shanshan;Yu, Qiuying;Zhao, Zheng;Li, Junwei;Ren, Hongtao;Wang, Na;Zhang, Gaiping
关键词:Salmonella typhimurium; Electrochemical immunosensor; Food safety; Metal -organic framework; Platinum nanoparticles; Multiwalled carbon nanotubes
-
Highly sensitive immunochromatographic assay for simultaneous determination of azaperone and azaperol in pork
作者:Chen, Linlin;Hu, Xiaofei;Xing, Yunrui;Sun, Yaning;Zhang, Gaiping;Chen, Linlin;Zhang, Gaiping;Chen, Linlin;Hu, Mei
关键词:Rapid detection; Azaperone; Azaperol; Monoclonal antibody; Immunoassay strip
-
Establishment of ultrasensitive immunoassay strip based on colloidal gold-McAb probe for detecting cyproheptadine hydrochloride and six phenothiazines in feed
作者:Hu, Xiaofei;Na, Guanqiong;Sun, Yaning;Xing, Yunrui;Zhang, Gaiping;Na, Guanqiong;Chen, Linlin;Zhang, Gaiping
关键词:Monoclonal antibody; Cyproheptadine; Phenothiazines; Immunochromatographic strip; Rapid detection
-
Isolation and Genome-Based Characterization of Biocontrol Potential of Bacillus siamensis YB-1631 against Wheat Crown Rot Caused by Fusarium pseudograminearum
作者:Dong, Qianqian;Liu, Qingxiang;Wu, Kun;Dong, Qianqian;Liu, Qingxiang;Deng, Xiaoxu;Xu, Wen;Xia, Mingcong;Zhang, Jie;Sun, Runhong;Wu, Chao;Yang, Lirong;Goodwin, Paul H.;Wang, Qi
关键词:Fusarium crown rot; Bacillus siamensis; Fusarium pseudograminearum; biocontrol; genome; growth promotion