文献类型： 外文期刊
作者： Qiu, Liang 1 ; Chen, Xing 1 ; Guo, Xiao-Meng 1 ; Gao, Wen 1 ; Zhao, Ruo-Heng 1 ; Zhang, Qing-Li 1 ; Yang, Bing 1 ; Huang, 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Lab Marine Fisheries Sci & Food Prod Proc,Yellow, Pilot Natl Lab Marine Sci & Technol Qingdao,Qingd, Key Lab Maricultural Organism Dis Control,Minist, Qingdao 266071, Peoples R China
2.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China
3.Shanghai Ocean Univ, Shanghai 201306, Peoples R China
关键词： Decapod iridescent virus 1; Shrimp hemocyte iridescent virus; Cherax quadricarinatus iridovirus; Real-time PCR; Shrimp
期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）
ISSN： 0022-2011
年卷期： 2020 年 173 卷
页码：
收录情况： SCI
摘要： Decapod iridescent virus 1 (DIV1) was proven to be the aetiological agent of a disease causing mass die-offs of shrimp, prawn and crayfish. The specific purpose of this study was to develop a new sensitive real-time PCR method for the specific detection of DIV1. A pair of primers that amplify a 142 bp fragment and a TaqMan probe were selected for the major capsid protein gene of DIV1. They were shown to be specific for DIV1 and did not react with other common shrimp pathogens or healthy shrimp DNA. The method could detect as virus levels as low as 1.2 copies of DIV1 plasmid DNA.
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