Improved Integrated Whole Proteomic and Phosphoproteomic Profiles of Severe Acute Pancreatitis
文献类型： 外文期刊
作者： Wang, Cheng 1 ; Zhang, Yanlei 2 ; Tan, Jinjuan 3 ; Chen, Bicheng 1 ; Sun, Linxiao 1 ;
作者机构： 1.Wenzhou Med Univ, Key Lab Diag & Treatment Severe Hepatopancreat Di, Zhejiang Prov Top Key Discipline Surg, Affiliated Hosp 1, Wenzhou 325000, Zhejiang, Peoples R China
2.Wenzhou Med Univ, Neurol Dept, Affiliated Hosp 1, Wenzhou 325000, Zhejiang, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Peoples R China
关键词： severe acute pancreatitis; proteomic; phosphoproteomic; DDA; DIA
期刊名称：JOURNAL OF PROTEOME RESEARCH （ 影响因子：4.466； 五年影响因子：4.352 ）
ISSN： 1535-3893
年卷期： 2020 年 19 卷 6 期
页码：
收录情况： SCI
摘要： Severe acute pancreatitis (SAP) is caused by complicated biological factors, and revealing its complex pathogenesis by single-target analysis is difficult. Systematic studies have developed slowly because extraction of degradable pancreatic proteins exposed to multiple proteases is challenging. We present integrated whole proteomic and phosphoproteomic profiles of SAP rats based on a modified protein extraction strategy with less protein degradation. Data-dependent acquisition (DDA) and data-independent acquisition (DIA) strategies were applied to select an appropriate method. Total 275 differentially expressed proteins and 757 differentially expressed phosphorylated proteins were identified by DIA-based quantitative proteomics. Several signal transduction pathways, including the AMPK, MAPK, and PI3K-Akt pathways, were enriched in SAP. Up-regulation of phosphorylated proteins involved in the process of TNFA signaling and inflammatory response was also detected in SAP. Our results improve the understanding of SAP development and progression.
- 相关文献
作者其他论文 更多>>
-
DIA-based phosphoproteomics profiling reveals rapid cold-responsive phosphorylation events in tomato fruit
作者：Tan, Jinjuan;Feng, Hanqian;Deng, Zhiping
关键词：Solanum lycopersicum; Proteomics; Phosphosites; Chilling injury; Postharvest
-
Integrated Metabolome and Microbiome Analysis Reveals the Regulatory Effects of Fermented Soybean Meal on the Gut Microbiota of Late Gestation
作者：Li, Yantao;Ren, Ying;Li, Yantao;Fu, Lele;Chen, Yushi;Yang, Hua;Wang, Cheng;Yang, Hua;Xiao, Yingping
关键词：gut microbiota; metabolite; fermentation; soybean meal; late gestation
-
An integrated proteomic and phosphoproteomic landscape of chronic kidney disease
作者：Sun, Linxiao;Wang, Cheng;Sun, Linxiao;Zhou, Zhongjing;Li, Qiangqiang
关键词：Chronic kidney disease; Unilateral ureteral obstruction; Proteomic; Phosphoproteomic; Oxidative phosphorylation; P -Jun
-
The kiwifruit amyloplast proteome (kfALP): a resource to better understand the mechanisms underlying amyloplast biogenesis and differentiation
作者：Li, Ang;Lin, Jiajia;Zeng, Zhebin;Chen, Xiaoya;Ding, Gang;Zhu, Man;Xu, Bin;Cheng, Yunjiang;Deng, Xiuxin;Zeng, Yunliu;Deng, Zhiping;Tan, Jinjuan;Atkinson, Ross G.;Nieuwenhuizen, Niels J.;Ampomah-Dwamena, Charles
关键词：Actinidia; amyloplast biogenesis; amyloplast differentiation; plastids; development; fruit quality; ripening
-
Bioconversion of soybean meal into gut microbiota-targeting polysaccharides via fermentation by Bacillus subtilis
作者：Wang, Cheng;Fu, Lele;Wang, Cheng;Zhang, Yu;Wei, Siyu;Wang, Yizhen;Jin, Mingliang;Zhang, Yu
关键词：Gut microbiota; Polysaccharides; Solid-state fermentation; Soybean meal; Transcriptome
-
Dissection of mRNA ac4C acetylation modifications in AC and Nr fruits: insights into the regulation of fruit ripening by ethylene
作者：Ma, Lili;Zheng, Yanyan;Bai, Chunmei;Wang, Qing;Zuo, Jinhua;Zhou, Zhongjing;Deng, Zhiping;Tan, Jinjuan;Fu, Anzhen
关键词：ac(4)C modification; Tomato; Ethylene; Fruit ripening; mRNA
-
The dynamic N1-methyladenosine RNA methylation provides insights into the tomato fruit ripening
作者：Ma, Lili;Zuo, Jinhua;Bai, Chunmei;Fu, Anzhen;Wang, Qing;Zheng, Yanyan;Ma, Lili;Bai, Chunmei;Zhou, Zhongjing;Deng, Zhiping;Tan, Jinjuan;Bouzayen, Mondher
关键词：m(1)A modification; tomato; fruit ripening; mRNA
