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Upregulation Sestrin2 protects against hydrogen peroxide-induced oxidative damage bovine mammary epithelial cells via a Keap1-Nrf2/ARE pathway

文献类型： 外文期刊

作者： Chen, Mengjiao 1 ; Xi, Yumeng 3 ; Chen, Kunlin 4 ; Xiao, Peng 2 ; Li, Shujie 1 ; Sun, Xiaochun 1 ; Han, Zhaoyu 1 ;

作者机构： 1.Nanjing Agr Univ, Inst Dairy Sci, Nanjing, Peoples R China

2.Guangxi Agr Vocat Tech Coll, Dept Anim Sci & Technol, Nanning, Peoples R China

3.Jiangsu Acad Agr Sci, Anim Husb Inst, Nanjing, Peoples R China

4.Jiangsu Acad Agr Sci, Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing, Peoples R China

关键词： Keap1-Nrf2; ARE pathway; MAC-T cells; oxidative stress; sestrin2

期刊名称：JOURNAL OF CELLULAR PHYSIOLOGY （ 影响因子：6.384； 五年影响因子：5.987 ）

ISSN： 0021-9541

年卷期：

页码：

收录情况： SCI

摘要： Sestrin2 (SESN2) is a highly conservative oxidative stress protein that can regulate energy metabolism, cell proliferation, apoptosis, and mitochondria autophagy processes. It plays a role as an antioxidant in various diseases. The aims of the present study were to explore the underlying role of SESN2 after hydrogen peroxide (H2O2) treatment in bovine mammary epithelial cells (MAC-T cells) by the methods of knockout or overexpression of SESN2. The results show that knockout of Sestrin2 exacerbate apoptosis, upregulate the expressions of Bax/Bcl2 in H2O2-treated MAC-T cells. Moreover, knockout of SESN2 also promoted reactive oxygen species (ROS) generation and exacerbated oxidative damage in H2O2-treated MAC-T cells. On the contrary, overexpression of SESN2 decreased apoptosis by downregulation of Bax/Bcl2 level decreased ROS generation and blocked oxidative damage in H2O2-treated MAC-T cells. In addition, results indicate that the Kelch-like ECH-associated protein-1 (Keap1)-nuclear factor (erythroid-derived 2) like2 (Nrf2)/antioxidant response element (ARE) signaling pathway was activated by H2O2; upregulation of SESN2 could relieve oxidative stress by inducing the expression of Keap1, Nrf2, HO-1, and NDPH: quinone oxidoreductase-1 protein. In conclusion, this study demonstrates that expression of SESN2 was significantly increased after H2O2 treatment and that SESN2 can alleviate oxidative stress and cell apoptosis in H2O2-treated MAC-T cells through activation of the Keap1-Nrf2/ARE pathway.

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