文献类型： 外文期刊
作者： Zhang, Fuliang 1 ; Luo, Jun 3 ; Teng, Man 3 ; Xing, Guangxu 3 ; Guo, Junqing 3 ; Zhang, Yihua 1 ;
作者机构： 1.Northwest A&F Univ, Coll Vet Med, 3 Taicheng Rd, Yangling 712100, Shaanxi, Peoples R China
2.Anyang Inst Technol, Coll Biol & Food Engn, Anyang, Peoples R China
3.Henan Acad Agr Sci, Minist Agr, Henan Prov Key Lab Anim Immunol, Key Lab Anim Immunol, Zhengzhou, Peoples R China
关键词： Capto Core 700; Japanese encephalitis virus; purification; Sepharose 4 fast flow; tangential-flow filtration
期刊名称：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY （ 影响因子：2.431； 五年影响因子：2.124 ）
ISSN： 0885-4513
年卷期：
页码：
收录情况： SCI
摘要： Purification of the enveloped virus poses a challenge as one must retain viral infectivity to preserve immunogenicity. The traditional process of virus purification is time-consuming, laborious and hard to scale up. Here, a rapid, simple and extensible laboratory program for the purification of Japanese encephalitis virus (JEV) was developed by using differential centrifugation, ultrafiltration, Sepharose 4 fast flow gel chromatography, and Capt Core 700 chromatography. The entire process recovered 61.64% of the original virus, and the purified virus particles maintained good activity and immunogenicity. The purification process described has potential application in large-scale production of high-purity JEV.
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