Thymol Protects Channel Catfish from Aeromonas hydrophila Infection by Inhibiting Aerolysin Expression and Biofilm Formation
文献类型： 外文期刊
作者： Dong, Jing 1 ; Zhang, Lushan 1 ; Liu, Yongtao 1 ; Xu, Ning 1 ; Zhou, Shun 1 ; Yang, Qiuhong 1 ; Yang, Yibin 1 ; Ai, Xiaoh 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
2.Minist Agr, Key Lab Control Qual & Safety Aquat Prod, Beijing 100071, Peoples R China
关键词： Aeromonas hydrophila; aerolysin; thymol; anti-virulence; biofilm
期刊名称：MICROORGANISMS （ 影响因子：4.128； ）
ISSN：
年卷期： 2020 年 8 卷 5 期
页码：
收录情况： SCI
摘要： Aeromonas hydrophila is an opportunistic pathogen responsible for a number of diseases in freshwater farming. Moreover, the bacterium has been identified as a zoonotic pathogen that threatens human health. Antibiotics are widely used for treatments of infectious diseases in aquaculture. However, the abuse of antibiotics has led to the emergence of antimicrobial resistant strains. Thus, novel strategies are required against resistant A. hydrophila strains. The quorum sensing (QS) system, involved in virulence factor production and biofilm formation, is a promising target in identifying novel drugs against A. hydrophila infections. In this study, we found that thymol, at sub-inhibitory concentrations, could significantly reduce the production of aerolysin and biofilm formation by inhibiting the transcription of genes aerA, ahyI, and ahyR. These results indicate that thymol inhibits the quorum sensing system. The protective effects of thymol against A. hydrophila mediated cell injury were determined by live/dead assay and lactate dehydrogenase (LDH) release assay. Moreover, the in vivo study showed that thymol could significantly decrease the mortality of channel catfish infected with A. hydrophila. Taken together, these findings demonstrate that thymol could be chosen as a phytotherapeutic candidate for inhibiting quorum sensing system-mediated aerolysin production and biofilm formation in A. hydrophila.
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