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Chloroplast development at low temperature requires the pseudouridine synthase gene TCD3 in rice

文献类型： 外文期刊

作者： Lin, Dongzhi 1 ; Kong, Rongrong 1 ; Chen, Lu 1 ; Wang, Yulu 1 ; Wu, Lanlan 1 ; Xu, Jianlong 2 ; Piao, Zhongze 3 ; Lee, Ga 1 ;

作者机构： 1.Shanghai Normal Univ, Coll Life Sci, Shanghai 200234, Peoples R China

2.Chinese Acad Agr Sci, Inst Crop Sci, 12 South Zhong Guan Cun St, Beijing 100081, Peoples R China

3.Shanghai Acad Agr Sci, Crop Breeding & Cultivat Res Inst, 1000 Jingqi Rd, Shanghai 201403, Peoples R China

4.Natl Inst Agr Sci, Jeon Ju 560500, South Korea

5.Shanghai Key Lab Plant Mol Sci, Shanghai 200234, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2020 年 10 卷 1 期

页码：

收录情况： SCI

摘要： Low temperature affects a broad spectrum of cellular components in plants, such as chloroplasts, as well as plant metabolism. On the other hand, pseudouridine (Psi) synthases are required for the most abundant post-transcriptional modification of RNA in Escherichia coli. However, the role of rice Psi synthases in regulating chloroplast development at low temperature remains elusive. In this study, we identified the rice thermo-sensitive chlorophyll-deficient (tcd3) mutant, which displays an albino phenotype before the 4-leaf stage and ultimately dies when grown at 20 degrees C, but can grow normally at 32 degrees C. Genetic analysis showed that the mutant trait is controlled by a single recessive nuclear gene (tcd3). Map-based cloning, complementation and knockout tests revealed that TCD3 encodes a chloroplast-localized Psi synthase. TCD3 is a cold-induced gene that is mainly expressed in leaves. The disruption of TCD3 severely affected the transcript levels of various chloroplast-associated genes, as well as ribosomal genes involved in chloroplast rRNA assembly at low temperature (20 degrees C), whereas the transcript levels of these genes were normal at high temperature (32 degrees C). These results provide a first glimpse into the importance of rice Psi synthase gene in chloroplast development at low temperatures.

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