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Development of a novel nano-based detection card by electrospinning for rapid and sensitive analysis of pesticide residues

文献类型： 外文期刊

作者： Zhai, Meng-Yu 1 ; Feng, Kun 1 ; Hu, Teng-Gen 2 ; Zong, Min-Hua 1 ; Wu, Hong 1 ;

作者机构： 1.South China Univ Technol, Sch Food Sci & Engn, Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Guangzhou 510640, Peoples R China

2.Guangdong Acad Agr Sci, Sericultural & Agri Food Res Inst, Key Lab Funct Foods, Minist Agr & Rural Affairs,Guangdong Key Lab Agr, Guangzhou, Peoples R China

关键词： rapid detection; pesticide residues; acetylcholinesterase; electrospinning

期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE （ 影响因子：3.638； 五年影响因子：3.802 ）

ISSN： 0022-5142

年卷期： 2020 年 100 卷 12 期

页码：

收录情况： SCI

摘要： BACKGROUND Increasing food safety awareness of consumers promotes the development of rapid and sensitive detection techniques for pesticide residues. In this study, a new type of rapid detection card for organophosphorus and carbamate pesticide residues was developed by electrospinning. The card involved enzyme fiber mat (EFM) and substrate fiber mat (SFM) which were prepared by mixing poly(vinyl alcohol) with acetylcholinesterase (AChE) and indolyl acetate (IA), respectively. RESULTS The mean diameter of fibers was 240 +/- 53 nm for EFM and 387 +/- 84 nm for SFM. Results of Fourier transform infrared and X-ray photoelectron spectroscopies confirmed that AChE and IA were successfully encapsulated into the fibers. The minimum concentrations of AChE and IA for effective detection were 1 and 3 mg mL(-1), respectively, and the optimal detection time was 15 min. The limits of detection for this card were 0.5 mg L(-1)for omethoate, 1.5 mg L(-1)for malathion, 0.1 mg L(-1)for carbaryl and 0.02 mg L(-1)for carbofuran. The detection card exhibited good storage stability and its activity could be maintained when stored at room temperature for at least 4 months. Additionally, the EFM can be reused three times. CONCLUSIONS The detection card obtained here was superior to a commercial card in detecting pesticide residues in real food samples. Hence, this electrospun detection card has potential for simple, rapid and sensitive analysis of pesticide residues. (c) 2020 Society of Chemical Industry

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