Quantitative Analysis of Spectinomycin and Lincomycin in Poultry Eggs by Accelerated Solvent Extraction Coupled with Gas Chromatography Tandem Mass Spectrometry
文献类型： 外文期刊
作者： Wang, Bo 1 ; Wang, Yajuan 2 ; Xie, Xing 4 ; Diao, Zhixiang 2 ; Xie, Kaizhou 2 ; Zhang, Genxi 2 ; Zhang, Tao 2 ; Dai, Guoj 1 ;
作者机构： 1.Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Jiangsu, Peoples R China
2.Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou 225009, Jiangsu, Peoples R China
3.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China
4.Minist Agr, Key Lab Vet Biol Engn & Technol, Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Peoples R China
关键词： poultry eggs; spectinomycin; lincomycin; ASE; GC-EI/MS/MS
期刊名称：FOODS （ 影响因子：4.35； 五年影响因子：4.957 ）
ISSN：
年卷期： 2020 年 9 卷 5 期
页码：
收录情况： SCI
摘要： A method based on accelerated solvent extraction (ASE) coupled with gas chromatography tandem mass spectrometry (GC-MS/MS) was developed for the quantitative analysis of spectinomycin and lincomycin in poultry egg (whole egg, albumen and yolk) samples. In this work, the samples were extracted and purified using an ASE350 instrument and solid-phase extraction (SPE) cartridges, and the parameters of the ASE method were experimentally optimized. The appropriate SPE cartridges were selected, and the conditions for the derivatization reaction were optimized. After derivatization, the poultry egg (whole egg, albumen and yolk) samples were analyzed by GC-MS/MS. This study used blank poultry egg (whole egg, albumen and yolk) samples to evaluate the specificity, sensitivity, linearity, recovery and precision of the method. The linearity (5.6-2000 mu g/kg for spectinomycin and 5.9-200 mu g/kg for lincomycin), correlation coefficient (>= 0.9991), recovery (80.0%-95.7%), precision (relative standard deviations, 1.0%-3.4%), limit of detection (2.3-4.3 mu g/kg) and limit of quantification (5.6-9.5 mu g/kg) of the method met the requirements for EU parameter verification. Compared with traditional liquid-liquid extraction methods, the proposed method is fast and consumes less reagents, and 24 samples can be processed at a time. Finally, the feasibility of the method was evaluated by testing real samples, and spectinomycin and lincomycin residues in poultry eggs were successfully detected.
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