A rapid novel visualized loop-mediated isothermal amplification method for Salmonella detection targeting at fimW gene
文献类型： 外文期刊
作者： Wen, Junping 1 ; Gou, Hongchao 2 ; Zhan, Zeqiang 1 ; Gao, Yuan 1 ; Chen, Zhengquan 1 ; Bai, Jie 1 ; Wang, Shaojun 1 ; Che 1 ;
作者机构： 1.South China Agr Univ, Coll Vet Med,Key Lab Zoonoses Prevent & Control G, Natl & Reg Joint Engn Lab Medicament Zoonoses Pre, Key Lab Zoonoses,Key Lab Anim Vaccine Dev,Minist, Guangzhou 510642, Peoples R China
2.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou, Peoples R China
关键词： Salmonella; fimW gene; LAMP; novel visualized detection
期刊名称：POULTRY SCIENCE （ 影响因子：3.352； 五年影响因子：3.679 ）
ISSN：
年卷期： 2020 年 99 卷 7 期
页码：
收录情况： SCI
摘要： Salmonella infection causes huge losses in the poultry industry worldwide. With the aim to prevent infectious diseases caused by Salmonella and to achieve rapid visualized Salmonella detection in poultry production, we used cresol red as an indicator to develop a novel visualized loop-mediated isothermal amplification method that targets the Salmonella fimW gene firstly in related field. The detection limit was 7.3 x 10(1) CFU/mL, and the method was highly specific and showed a high clinical detection rate. The entire reaction can be completed in about 40 min and only requires a water bath at 62 degrees C, which makes the method extremely suitable for application to poultry production.
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