您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Involvement of the chloroplast gene ferredoxin 1 in multiple responses of Nicotiana benthamiana to Potato virus X infection

文献类型： 外文期刊

作者： Yang, Xue 1 ; Lu, Yuwen 1 ; Wang, Fang 4 ; Chen, Ying 3 ; Tian, Yanzhen 5 ; Jiang, Liangliang 1 ; Peng, Jiejun 1 ; Zheng, 1 ;

作者机构： 1.Ningbo Univ, Inst Plant Virol, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Key Lab Biotechnol Plant Protect MOA & Zhejiang P, Hangzhou 310021, Peoples R China

3.Shenyang Agr Univ, Dept Plant Protect, Shenyang 110161, Peoples R China

4.Ningbo Acad Agr Sci, Ningbo 315211, Peoples R China

5.Fujian Agr & Forestry Univ, Coll Plant Protect, Fuzhou 350002, Peoples R China

6.James Hutton Inst, Cell & Mol Sci Grp, Dundee DD2 5DA, Scotland

7.RAS, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

关键词： ABA; ferredoxin 1; p25; PD callose; Potato virus X; SA

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2020 年 71 卷 6 期

页码：

收录情况： SCI

摘要： The chloroplast protein ferredoxin 1 (FD1), with roles in the chloroplast electron transport chain, is known to interact with the coat proteins (CPs) of Tomato mosaic virus and Cucumber mosaic virus. However, our understanding of the roles of FD1 in virus infection remains limited. Here, we report that the Potato virus X (PVX) p25 protein interacts with FD1, whose mRNA and protein levels are reduced by PVX infection or by transient expression of p25. Silencing of FD1 by Tobacco rattle virus-based virus-induced gene silencing (VIGS) promoted the local and systemic infection of plants by PVX. Use of a drop-and-see (DANS) assay and callose staining revealed that the permeability of plasmodesmata (PDs) was increased in FD1-silenced plants together with a consistently reduced level of PD callose deposition. After FD1 silencing, quantitative reverse transcription-real-time PCR (qRT-PCR) analysis and LC-MS revealed these plants to have a low accumulation of the phytohormones abscisic acid (ABA) and salicylic acid (SA), which contributed to the decreased callose deposition at PDs. Overexpression of FD1 in transgenic plants manifested resistance to PVX infection, but the contents of ABA and SA, and the PD callose deposition were not increased in transgenic plants. Overexpression of FD1 interfered with the RNA silencing suppressor function of p25. These results demonstrate that interfering with FD1 function causes abnormal plant hormone-mediated antiviral processes and thus enhances PVX infection.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台