cDNA-AFLP analysis of salicylic acid- and calcium chloride-induced transcript derived fragments under drought in tomato (Solanum lycopersicum)
文献类型： 外文期刊
作者： Zhang, Dongye 1 ; Xie, Libo 2 ; Xu, Xiangyang 1 ;
作者机构： 1.Northeast Agr Univ, Coll Hort & Landscape Architecture, Key Lab Biol & Genet Improvement Hort Crops North, Lab Genet Breeding Tomato,Minist Agr & Rural Affa, Harbin, Heilongjiang, Peoples R China
2.Heilongjiang Acad Agr Sci, Hort Subacad, Lab Biotechnol, Harbin, Heilongjiang, Peoples R China
关键词： cDNA-AFLP; differentially expressed transcripts; drought stress; exogenous salicylic acid and calcium chloride; tomato
期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）
ISSN： 1310-2818
年卷期： 2020 年 34 卷 1 期
页码：
收录情况： SCI
摘要： Drought seriously threatens tomato production worldwide. Despite much research on exogenous salicylic acid (SA) and Ca(2+)improving plant resistance to biotic and abiotic stress, the molecular mechanisms of exogenous SA- and Ca2+-mediated drought resistance response in tomato remain unclear. In this study, we analyzed SA- and Ca2+-induced differentially expressed transcripts under drought in tomato plants using cDNA-amplified fragment length polymorphism (cDNA-AFLP). In total, 34 transcript derived fragments (TDFs) were differentially expressed. The functions identified through NCBI BLAST alignment mainly involved signal transduction, amino acid metabolism, transcription factors, transfer transport and stress response. The quantitative real-time polymerase chain reaction results of 12 TDFs associated with drought response were consistent with the patterns of changes observed with cDNA-AFLP analysis. These differentially expressed transcripts may be used for functional verification, transgenic research and breeding of drought-resistant tomato varieties.
- 相关文献
作者其他论文 更多>>
-
Mapping of the Cladosporium fulvum resistance gene Cf-16, a major gene involved in leaf mold disease in tomato
作者：Zhang, Dongye;Liu, Guan;Feng, Guojun;Li, Huijia;Xu, Xiangyang;Xie, Libo
关键词：tomato; mapping; Cladosporium fulvum; resistance gene Cf-16; BSA; SSR markers
-
Combining Transcriptomics and Metabolomics to Uncover the Effects of High-Energy Lithium-Ion Beam Irradiation on Capsicum annuum L.
作者：Xie, Libo;Wang, Xue;Xu, Chunmei;Guo, Mu;Liu, Luxiang;Xie, Yongdun;Xiong, Hongchun;Han, Xinchun
关键词：Capsicum annuum L.; physiology; transcriptomics; metabolomics; lithium-ion beam
-
Comparative transcriptome analysis reveals the response mechanism of Cf-16-mediated resistance to Cladosporium fulvum infection in tomato
作者：Zhang, Dongye;Bao, Yufang;Sun, Yaoguang;Yang, Huanhuan;Zhao, Tingting;Li, Huijia;Du, Chong;Jiang, Jingbin;Li, Jingfu;Xu, Xiangyang;Xie, Libo
关键词：Comparative transcriptome; Cladosporium fulvum; Cf-16 tomato; Molecular mechanism
-
BIOLOGICAL EFFECTS OF 7LITHIUM ((LI)-L-7) ION BEAM RADIATION ON MUTATION INDUCTION IN CAPSICUM ANNUUM L.
作者：Wang, Xue;Xie, Libo;Chen, Lixin;Zhang, Hui;Liu, Luxiang
关键词：Capsicum annuum L.; Li-7 ion beam; Mutant; SRAP
-
Isolation and detection of differential genes in hot pepper (Capsicum annuum L.) after space flight using AFLP markers
作者：Xie, Libo;Wang, Xue;Zhou, Yu;Chen, Lixin;Gao, Yongli;Guo, Yahua;Peng, Mu;Meng, Fanjuan;Liu, Luxiang
关键词：Hot pepper (Capsicum annuum L.);Space-inducement;AFLP
