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Multigene engineering of medium-chain fatty acid biosynthesis in transgenicArabidopsis thalianaby aCre/LoxPmultigene expression system

文献类型： 外文期刊

作者： Zheng, Yusheng 1 ; Chen, Lizhi 1 ; Zhu, Zhiyong 1 ; Li, Dongdong 1 ; Zhou, Peng 2 ;

作者机构： 1.Hainan Univ, Coll Trop Crops, Haikou 570228, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

关键词： Medium-chain fatty acids (MCFAs); CnKASI; CnFatB3; CnLPAAT-B; Combinatorial expression effect; Cre; loxcombination system

期刊名称：3 BIOTECH （ 影响因子：2.406； 五年影响因子：3.203 ）

ISSN： 2190-572X

年卷期： 2020 年 10 卷 8 期

页码：

收录情况： SCI

摘要： Medium-chain fatty acids (MCFAs) are an ideal feedstock for biodiesel and a range of oleochemical products. In this study, different combinations ofCnFATB3,CnLPAAT-BandCnKASIfrom coconut (Cocos nuciferaL.) were coexpressed in transgenicArabidopsis thaliana bya Cre/LoxP multigene expression system. Transgenic lines expressing different combinations of these genes were designated FL (FatB3 + LPAAT-B), FK (FatB3 + KASI) and FLK (FatB3 + LPAAT-B + KASI). The homozygous seeds of transgenicArabidopsis thalianaexpressing high levels of these genes were screened, and their fatty acid composition and lipid contents were determined. Compared with its content in wild-typeA. thaliana, the lauric acid (C12:0) content was significantly increased by at least 395%, 134% and 124% in FLK, FL and FK seeds, respectively. Meanwhile, the myristic acid (C14:0) content was significantly increased by at least 383%, 106% and 102% in FL, FLK and FK seeds, respectively, compared to its level in wild-type seeds. Therefore, the FLK plants exhibited the best effects to increase the level of C12:0, and FL expressed the optimal combination of genes to increase the level of 14:0 MCFA.

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