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Novel Soil Bacterium StrainDesulfitobacteriumsp. PGC-3-9 Detoxifies Trichothecene Mycotoxins in Wheat via De-Epoxidation under Aerobic and Anaerobic Conditions

文献类型： 外文期刊

作者： He, Wei-Jie 1 ; Shi, Meng-Meng 2 ; Yang, Peng 2 ; Huang, Tao 2 ; Yuan, Qing-Song 2 ; Yi, Shu-Yuan 2 ; Wu, Ai-Bo 5 ; Li, H 1 ;

作者机构： 1.Hubei Acad Agr Sci, Wheat Dis Biol Res Stn Cent China, Hubei Key Lab Food Crop Germplasm & Genet Improve, Hubei Engn & Technol Res Ctr Wheat,Food Crops Ins, Wuhan 430064, Peoples R China

2.Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China

3.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China

4.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China

5.Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Food Safety Res Inst Nutr Sci, Shanghai 200031, Peoples R China

关键词： trichothecene; deoxynivalenol; nivalenol; de-epoxidation; Desulfitobacterium; aerobic and anaerobic detoxification; wheat

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN：

年卷期： 2020 年 12 卷 6 期

页码：

收录情况： SCI

摘要： Trichothecenes are the most common mycotoxins contaminating small grain cereals worldwide. The C12,13 epoxide group in the trichothecenes was identified as a toxic group posing harm to humans, farm animals, and plants. Aerobic biological de-epoxidation is considered the ideal method of controlling these types of mycotoxins. In this study, we isolated a novel trichothecene mycotoxin-de-epoxidating bacterium,Desulfitobacteriumsp. PGC-3-9, from a consortium obtained from the soil of a wheat field known for the occurrence of frequentFusariumhead blight epidemics under aerobic conditions. Along with MMYPF media, a combination of two antibiotics (sulfadiazine and trimethoprim) substantially increased the relative abundance ofDesulfitobacteriumspecies from 1.55% (aerobic) to 29.11% (aerobic) and 28.63% (anaerobic). A single colony purified strain, PGC-3-9, was isolated and a 16S rRNA sequencing analysis determined that it wasDesulfitobacterium. The PGC-3-9 strain completely de-epoxidated HT-2, deoxynivalenol (DON), nivalenol and 15-acetyl deoxynivalenol, and efficiently eliminated DON in wheat grains under aerobic and anaerobic conditions. The strain PGC-3-9 exhibited high DON de-epoxidation activity at a wide range of pH (6-10) and temperature (15-50 degrees C) values under both conditions. This strain may be used for the development of detoxification agents in the agriculture and feed industries and the isolation of de-epoxidation enzymes.

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