In vitrodisinfection efficacy and clinical protective effects of common disinfectants against acute hepatopancreatic necrosis disease (AHPND)-causingVibrioisolates in Pacific white shrimpPenaeus vannamei
文献类型： 外文期刊
作者： Zou, Peizhuo 1 ; Yang, Qian 1 ; Wang, Hailiang 1 ; Xie, Guosi 1 ; Cao, Zhi 1 ; Chen, Xing 1 ; Gao, Wen 1 ; Huang, Jie 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Lab Marine Fisheries Sci & Food Prod Proc,Qingdao, Qingdao Key Lab Mariculture Epidemiol & Biosecuri, Minist Agr & Rural Affairs,Key Lab Maricultural O, Qingdao 266071, Peoples R China
2.Univ Ghent, Ctr Microbial Ecol & Technol CMET, Coupure Links 653, B-9000 Ghent, Belgium
关键词： AHPND; disinfectants; disinfection efficacy; toxicity; protection activity
期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）
ISSN： 1225-8873
年卷期： 2020 年 58 卷 8 期
页码：
收录情况： SCI
摘要： Acute hepatopancreatic necrosis disease (AHPND) is one of the most significant bacterial diseases in global shrimp culture, causing severe economic losses. In the present study, we carried outin vitroantimicrobial tests to investigate the disinfection efficacy of 14 common disinfectants toward different AHPND-causingVibriospp., including eight isolates ofV. parahaemolyticus, four isolates ofV. campbellii, and one isolate ofV. owensii. Polyhexamethylene biguanidine hydrochloride (PHMB) was revealed to possess the strongest inhibitory activity. Through analyzing and evaluating the results of antimicrobial tests and acute toxicity test, we selected PHMB and hydrogen peroxide (H2O2) for further clinical protection test. Clinical manifestations indicated that both PHMB (2 mg/L and 4 mg/L) and H2O2(12 mg/L) could effectively protect juvenilePenaeus vannameifrom the infection ofV. parahaemolyticusisolateVp362 at 10(6)CFU/ml, and the survival rate was over 80%. When the bacterial concentration was reduced to 10(5)CFU/ml, 10(4)CFU/ml, and 10(3)CFU/ml, the survival rate after treated by 1 mg/L PHMB was 64.44%, 93.33%, and 100%, respectively. According to the results, PHMB and H(2)O(2)showed a lower toxicity while a better protection activity, particularly against a lower concentration of the pathogens. Therefore, these two disinfectants are proved to be promising disinfectants that can be applied to prevent and control AHPND in shrimp culture. Moreover, the methods of this study also provided valuable information for the prevention of other important bacterial diseases and suggested a reliable means for screening potential drugs in aquaculture.
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