Evaluating the effect ofTLR4-overexpressing on the transcriptome profile in ovine peripheral blood mononuclear cells
文献类型： 外文期刊
作者： Guo, Xiaofei 1 ; Zhang, Jinlong 1 ; Li, Yao 2 ; Yang, Jing 1 ; Li, Yihai 1 ; Dong, Chunxiao 1 ; Liu, Guoshi 2 ; Lian, Zhe 1 ;
作者机构： 1.Tianjin Acad Agr Sci, Tianjin Inst Anim Husb & Vet Med, Tianjin 300381, Peoples R China
2.China Agr Univ, Coll Anim Sci & Technol, Beijing 100193, Peoples R China
关键词： TLR4-overexpressing; Peripheral blood mononuclear cells; Transcriptome; Sheep
期刊名称：JOURNAL OF BIOLOGICAL RESEARCH-THESSALONIKI （ 影响因子：1.889； 五年影响因子：2.988 ）
ISSN： 1790-045X
年卷期： 2020 年 27 卷 1 期
页码：
收录情况： SCI
摘要： Background Toll-like receptor 4 (TLR4) plays an important role in the elimination of Gram-negative bacteria infections and the initiation of antiinflammatory response. Using the technology of pronuclear microinjection, genetically modified (GM) sheep withTLR4overexpression were generated. Previous studies have shown that these GM sheep exhibited a higher inflammatory response to Gram-negative bacteria infection than wild type (WT) sheep. In order to evaluate the gene expression of GM sheep and study the co-expressed and downstream genes forTLR4, peripheral blood mononuclear cells (PBMC) fromTLR4-overexpressing (Tg) and wild type (WT) sheep were selected to discover the transcriptomic differences using RNA-Seq. Result An average of 18,754 and 19,530 known genes were identified in the Tg and WT libraries, respectively. A total of 338 known genes and 85 novel transcripts were found to be differentially expressed in the two libraries (p < 0.01). A differentially expressed genes (DEGs) enrichment analysis showed that the GO terms of inflammatory response, cell recognition, etc. were significantly (FDR < 0.05) enriched. Furthermore, the above DEGs were significantly (FDR < 0.05) enriched in the sole KEGG pathway of the Phagosome. Real-time PCR showed theOLR1,TLR4andCD14genes to be differentially expressed in the two groups, which validated the DEGs data. Conclusions The RNA-Seq results revealed that the overexpressedTLR4in our experiment strengthened the ovine innate immune response by increasing the phagocytosis in PBMC.
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