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Transcriptome analysis provides insights into the non-methylated lignin synthesis in Paphiopedilum armeniacum seed

文献类型： 外文期刊

作者： Fang, Lin 1 ; Xu, Xin 1 ; Li, Ji 1 ; Zheng, Feng 1 ; Li, Mingzhi 1 ; Yan, Jingwei 3 ; Li, Yuan 1 ; Zhang, Xinhua 1 ; Li, Lin 1 ;

作者机构： 1.Chinese Acad Sci, Guangdong Prov Key Lab Appl Bot, South China Bot Garden, Guangzhou 510650, Peoples R China

2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

3.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China

4.Guangdong Acad Agr Sci, Environm Hort Res Inst, Guangzhou 510640, Peoples R China

5.Chinese Acad Sci, Key Lab South China Agr Plant Mol Anal & Gene Imp, South China Bot Garden, Guangzhou 510650, Peoples R China

关键词： Paphiopedilum armeniacum; Lignin; Transcriptome analysis; Germination

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2020 年 21 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundsPaphiopedilum is an important genus of the orchid family Orchidaceae and has high horticultural value. The wild populations are under threat of extinction because of overcollection and habitat destruction. Mature seeds of most Paphiopedilum species are difficult to germinate, which severely restricts their germplasm conservation and commercial production. The factors inhibiting germination are largely unknown.ResultsIn this study, large amounts of non-methylated lignin accumulated during seed maturation of Paphiopedilum armeniacum (P. armeniacum), which negatively correlates with the germination rate. The transcriptome profiles of P. armeniacum seed at different development stages were compared to explore the molecular clues for non-methylated lignin synthesis. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that a large number of genes associated with phenylpropanoid biosynthesis and phenylalanine metabolism during seed maturation were differentially expressed. Several key genes in the lignin biosynthetic pathway displayed different expression patterns during the lignification process. PAL, 4CL, HCT, and CSE upregulation was associated with C and H lignin accumulation. The expression of CCoAOMT, F5H, and COMT were maintained at a low level or down-regulated to inhibit the conversion to the typical G and S lignin. Quantitative real-time RT-PCR analysis confirmed the altered expression levels of these genes in seeds and vegetative tissues.ConclusionsThis work demonstrated the plasticity of natural lignin polymer assembly in seed and provided a better understanding of the molecular mechanism of seed-specific lignification process.

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