Identification and comparative expression analysis of RIG-I and MDA5 in Chinese giant salamanderAndrias davidianus
文献类型： 外文期刊
作者： Meng, Yan 1 ; Fan, Yuding 1 ; Zhou, Yong 1 ; Jiang, Nan 1 ; Xue, Mingyang 1 ; Liu, Wenzhi 1 ; Li, Yiqun 1 ; Zeng, Lingbin 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Fish Dis Div, Wuhan 430223, Hubei, Peoples R China
关键词： Andrias davidianus; antiviral responses; Chinese giant salamander; gene expression; GSIV infection; MDA5; qPCR; RIG-I
期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）
ISSN： 1355-557X
年卷期： 2020 年 51 卷 11 期
页码：
收录情况： SCI
摘要： The cytoplasmic helicases retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated protein 5 (MDA5) are two pattern recognition receptor genes belonging to RIG-I-like receptor (RLR). They play a crucial role in host sensing of pathogen invasion. Herein, we identified and characterized complete cDNA of RIG-I and MDA5 in Chinese giant salamander (adRIG-I and adMDA5). To evaluate their expression levels, real-time fluorescence quantitative RT-PCR (qPCR) was performed between normal and Chinese giant salamander iridovirus (GSIV)-infected tissues. The full-length adMDA5 cDNA of 3,509 bp encoded a 968-acid amino polypeptide, and the 3567-bp adRIG-I cDNA encoded a 951-acid amino protein. The amino acid sequence analysis revealed adRIG-I and adMDA5 kept the conserved domains of RLR, and the two genes both shared relatively high similarity homologs with other species. Two genes were expressed in most tissues ofAndrias davidianus, with the highest expression in spleen. After GSIV infection, both adRIG-I and adMDA5 transcripts were initially increased then decreased in kidney and spleen. These results suggest that adRIG-I and adMDA5 can response to GSIV infection and may play a role in antiviral infections in DNA virus.
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