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Characteristics of Circular RNA Expression Profiles of Porcine Granulosa Cells in Healthy and Atretic Antral Follicles

文献类型： 外文期刊

作者： Meng, Li 1 ; Teerds, Katja 7 ; Tao, Jian 1 ; Wei, Hengxi 1 ; Jaklofsky, Marcel 7 ; Zhao, Zhihong 1 ; Liang, Yaodi 1 ; Li, 1 ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou 510642, Peoples R China

2.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Minist Agr, Guangzhou 510642, Peoples R China

3.South China Agr Univ, Key Lab Chicken Genet Breeding & Reprod, Minist Agr, Guangzhou 510642, Peoples R China

4.Chinese Univ Hong Kong, Dept Obstet & Gynaecol, Shatin, Hong Kong, Peoples R China

5.Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci, Shatin, Hong Kong, Peoples R China

6.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China

7.Wageningen Univ, Human & Anim Physiol, De Elst 1, NL-6708 WD Wageningen, Netherlands

关键词： circRNA expression profiles; antral follicular atresia; granulosa cell apoptosis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2020 年 21 卷 15 期

页码：

收录情况： SCI

摘要： Circular RNAs (circRNAs) are thought to play essential roles in multiple biological processes, including apoptosis, an important process in antral follicle atresia. We aimed to investigate the potential involvement of circRNAs in granulosa cell apoptosis and thus antral follicle atresia. CircRNA expression profiles were generated from porcine granulosa cells isolated from healthy antral (HA) and atretic antral (AA) follicles. Over 9632 circRNAs were identified, of which 62 circRNAs were differentially expressed (DE-circRNAs). Back-splicing, RNase R resistance, and stability of DE-circRNAs were validated, and miRNA binding sites and related target genes were predicted. Two exonic circRNAs with low false discovery rate (FDR) high fold change, miRNA binding sites, and relevant biological functions-circ_CBFA2T2 and circ_KIF16B-were selected for further characterization. qRT-PCR and linear regression analysis confirmed expression and correlation of the targeted genes-the antioxidant geneGCLC(potential target of circ_CBFA2T2) and the apoptotic geneTP53(potential target of circ_KIF16B). Increased mRNA content ofTP53in granulosa cells of AA follicles was further confirmed by strong immunostaining of both p53 and its downstream target pleckstrin homology like domain family a member 3 (PHLDA3) in AA follicles compared to negligible staining in granulosa cells of HA follicles. Therefore, we concluded that aberrantly expressed circRNAs presumably play a potential role in antral follicular atresia.

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