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Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes)

文献类型： 外文期刊

作者： Fan, Xiu-Zhi 1 ; Yao, Fen 1 ; Yin, Chao-Min 1 ; Shi, De-Fang 1 ; Gao, Hong 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Agroprod Proc & Nucl Agr Technol, Wuhan 430064, Peoples R China

关键词： Auricularia auricula-judae; intracellular polysaccharide; submerged fermentation; response surface methodology; quantitative reverse transcription PCR; medicinal mushrooms

期刊名称：INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS （ 影响因子：1.921； 五年影响因子：1.879 ）

ISSN： 1521-9437

年卷期： 2020 年 22 卷 6 期

页码：

收录情况： SCI

摘要： To improve the production of mycelial biomass and intracellular polysaccharide (IPS), the culture medium composition and culture condition were respectively optimized by response surface methodology and one-factor-at-a-time methodology. The impact of fermentation process on the transcription of genes involved in IPS synthesis was eval-uated using quantitative reverse transcription PCR (qRT-PCR). The yield of biomass and IPS were increased by 150% and 177% under the optimized fermentation process, respectively. Moreover, the optimization of fermentation process significantly increased the transcription of genes involved in IPS synthesis, especially pgm-1, ugp, and pgi. This is the first exploration of gene transcription profile for IPS synthesis of Auricularia auricula-judae under submerged fermentation. These results could provide a potential tool for fermentation process optimization and transcriptional regulation of IPS biosynthesis in A. auricula-judae.

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