First report and genetic characterization of bovine torovirus in diarrhoeic calves in China
文献类型: 外文期刊
作者: Shi, Zhihai 1 ; Wang, Wenjia 3 ; Chen, Chaoxi 4 ; Zhang, Xiaozhan 3 ; Wang, Jing 1 ; Xu, Zhaoxue 1 ; Lan, Yali 1 ;
作者机构: 1.Henan Acad Agr Sci, Inst Anim Husb & Vet Med, Zhengzhou 450002, Henan, Peoples R China
2.Henan Key Lab Farm Anim Breeding & Nutr Regulat, Zhengzhou 450002, Henan, Peoples R China
3.Henan Univ Anim Husb & Econ, Coll Vet Med & Pharmaceut Engn, Zhengzhou 450046, Henan, Peoples R China
4.Southwest Univ Nationalities, Coll Life Sci & Technol, Chengdu 610041, Sichuan, Peoples R China
关键词: Bovine torovirus; China; Calf diarrhoea; Beef; Dairy; Phylogenetic analysis
期刊名称:BMC VETERINARY RESEARCH ( 影响因子:2.741; 五年影响因子:2.955 )
ISSN:
年卷期: 2020 年 16 卷 1 期
页码:
收录情况: SCI
摘要: Background Coronaviruses are notorious pathogens that cause diarrheic and respiratory diseases in humans and animals. Although the epidemiology and pathogenicity of coronaviruses have gained substantial attention, little is known about bovine coronavirus in cattle, which possesses a close relationship with human coronavirus. Bovine torovirus (BToV) is a newly identified relevant pathogen associated with cattle diarrhoea and respiratory diseases, and its epidemiology in the Chinese cattle industry remains unknown. Results In this study, a total of 461 diarrhoeic faecal samples were collected from 38 different farms in three intensive cattle farming regions and analysed. Our results demonstrated that BToV is present in China, with a low prevalence rate of 1.74% (8/461). The full-length spike genes were further cloned from eight clinical samples (five farms in Henan Province). Phylogenetic analysis showed that two different subclades of BToV strains are circulating in China. Meanwhile, the three BToV strains identified from dairy calves, 18,307, 2YY and 5YY, all contained the amino acid variants R614Q, I801T, N841S and Q885E. Conclusions This is the first report to confirm the presence of BToV in beef and dairy calves in China with diarrhea, which extend our understanding of the epidemiology of BToVs worldwide.
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