文献类型： 外文期刊
作者： Ding, Baojuan 1 ; Zhang, Xiaoyan 1 ; Xu, Yongsheng 1 ; An, Lijia 1 ; Liu, Xiangguo 2 ; Su, Qiao 1 ;
作者机构： 1.Dalian Univ Technol, Sch Bioengn, Dalian, Peoples R China
2.Jilin Acad Agr Sci, Inst Agr Biotechnol, Changchun, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2020 年 15 卷 8 期
页码：
收录情况： SCI
摘要： K(+)is an essential nutrient for plant growth and is responsible for many important physiological processes. K(+)deficiency leads to crop yield losses, and overexpression of K(+)transporter genes has been proven to be an effective way to resolve this problem. However, current research on the overexpression of K(+)transporter genes is limited to plant sources. TrkH is a bacterial K(+)transporter whose function generally depends on the regulation of TrkA. To date, whether TrkH can improve K(+)uptake in eukaryotic organisms is still unknown. In this study, a novelMbtrkHgene was cloned from marine microbial metagenomic DNA. Functional complementation and K+-depletion analyses revealed that MbTrkH functions in K(+)uptake in the K+-deficient yeast strain CY162. Moreover, K+-depletion assays revealed thatMbtrkHoverexpression improves plant K(+)uptake. K(+)hydroponic culture experiments showed that, compared with WT tobacco lines,MbtrkHtransgenic tobacco lines had significantly greater fresh weights, dry weights and K(+)contents. These results indicate that MbTrkH promotes K(+)uptake independently of TrkA in eukaryotes and provide a new strategy for improving K+-use efficiency in plants.
- 相关文献
作者其他论文 更多>>
-
Powerful combination: a genome editing system to improve efficiency of breeding inducer and haploid sorting in maize
作者：Xia, Hanchao;Yin, Yuejia;Zhang, Chuang;Chen, Ziqi;Liu, Xiangguo;Xia, Hanchao;Zhang, Di;Zhao, Rengui;Liu, Xiangguo;Qu, Yanzhi;Jiang, Shurong;Wang, Xinqi;Xu, Jieting
关键词：haploid; MoDsRed2; inducer line; genome-editing; Zea mays
-
CRISPR/dCas9-assisted SERS sensing for transgenic crops based on two-factor verification of 35S promoter and Nos terminator
作者：Su, Ailing;Xu, Weiqing;Xu, Shuping;Chen, Ziqi;Liu, Xiangguo;Liang, Chongyang;Xu, Shuping;Xu, Shuping
关键词：CRISPR/dCas9; SERS; Gene detection; Transgenic crop identification; Multiplex detection
-
CRISPR/Cas-SERS Sensing Platforms: A Frontier Technology for Next-Generation Fast, Low-Cost, Ultra-Micro Biosample Detection
作者：Bao, Chengxin;Xu, Shuping;Liu, Xiangguo;Liang, Chongyang;Xu, Shuping
关键词：CRISPR/Cas detection; nucleic acid analysis; SERS detection;
sg RNA -
LcTprxII Overexpression Enhances Physiological and Biochemical Effects in Maize Under Alkaline (Na2CO3) Stress
作者：Michael, David Pitia Julius;Lu, Jainyu;Rehman, Faiz Ur;Temitope, Aroge;Qian, Buxuan;Liu, Xiangguo;Qi, Xin;Sun, Ruidong;Chen, Ziqi;Zhang, Jian;Liu, Qing;Wei, Xuancheng;Xia, Hanchao;Han, Jiarui;Liu, Xiangguo;Chen, Ziqi;Yin, Yuejia;Jiang, Long
关键词：LcTprxII; alkaline stress; type II peroxiredoxin; transcriptome; hormone regulation
-
Amplification-free detection of genetically modified crops via spatial confinement CHA/HCR-aided surface-enhanced resonance Raman spectroscopy
作者：Bao, Chengxin;Wang, Huimin;Liu, Lin;Xu, Shuping;Li, Feiwu;He, Yuxuan;Yin, Yuejia;Liu, Xiangguo;Liu, Lin;Chang, Jingjing;Ding, Lan;Xu, Shuping;Xu, Shuping
关键词：Amplification-free; CHA; HCR; Spatial confinement; SERS
-
Isolation and Functional Analysis of Na+/H+ Antiporter Gene (LcNHX1) from Leymus chinensis
作者：Sun, Chuanbo;Zhang, Chuang;Yin, Yuejia;Mu, Shujing;Liu, Qing;Wang, Ziyu;Liu, Yang;Zhang, Chunxiao;Liu, Xiangguo;Zhang, Yan;Guo, Jia;Wang, Ying
关键词：NHX1; Leymus chinensis; Saline-alkali tolerance; Candidate gene
-
Genetic variation in the aquaporin TONOPLAST INTRINSIC PROTEIN 4;3 modulates maize cold tolerance
作者：Zeng, Rong;Zhang, Xiaoyan;Li, Minze;Fu, Diyi;Zhang, Zhuo;Gao, Lei;Zhang, Shuaisong;Yang, Xiaohong;Tian, Feng;Yang, Shuhua;Shi, Yiting;Song, Guangshu;Lv, Qingxue;Yang, Xiaohong;Tian, Feng
关键词：cold stress; maize; TIP4;3; natural variation; stomatal movement; reactive oxygen species
