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Integrative Proteomic and MicroRNA Analysis: Insights Into Mechanisms of Eyestalk Ablation-Induced Ovarian Maturation in the Swimming CrabPortunus trituberculatus

文献类型： 外文期刊

作者： Meng, Xianliang 1 ; Zhang, Mengqian 3 ; Gao, Baoquan 2 ; Lv, Jianjian 2 ; Li, Jian 2 ; Liu, Ping 2 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Aquat Genom, Minist Agr & Rural Affairs, Qingdao, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr & Rural Affairs, Qingdao, Peoples R China

4.Jiangsu Ocean Univ, Coll Marine Life & Fisheries, Lianyungang, Peoples R China

关键词： crab; eyestalk ablation; miRNA; proteome; ovarian development

期刊名称：FRONTIERS IN ENDOCRINOLOGY （ 影响因子：5.555； 五年影响因子：5.456 ）

ISSN： 1664-2392

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： Eyestalk ablation is the most common method to induce ovarian maturation in decapod crustacean aquaculture, but it jeopardizes broodstock survival and larvae production. It is important to understand the molecular basis underlying the maturation triggered by ablation and thereby develop an alternative measure for maturation manipulation. In this study, we investigate alterations of ovarian proteome and miRNA profile after ablation in a commercially important marine crabPortunus trituberculatus. Quantitative proteomic analysis using iTRAQ reveals that 163 proteins are differentially expressed following ablation, and modulation of methyl farnesoate metabolism and activation of calcium signaling may play important roles in the ovarian maturation induced by ablation. miRNA expression profiling identifies 31 miRNAs that show statistically significant changes. Integration of miRNA and proteome expression data with miRNA target prediction algorithms generates a potential regulatory network consisting of 26 miRNAs and 30 proteins linked by 71 possible functional associations. The miRNA-protein network analysis suggests that miRNAs are involved in promoting ovarian maturation by controlling expression of proteins related to methyl farnesoate synthesis, calcium signals, and energy metabolism. Experimental validation and temporal expression analysis indicate multiple miRNAs can act synergistically to regulate expression of Farnesoic acid O-methyltransferase and Calmodulin. Our findings provide new insights for elucidating the mechanisms underlying eyestalk ablation-induced ovarian maturation and could be useful for devising an alternative technique for manipulating reproduction inP. trituberculatusand other decapods.

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