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The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete Peronophythora litchii

文献类型： 外文期刊

作者： Kong, Guanghui 1 ; Chen, Yubin 1 ; Deng, Yizhen 1 ; Feng, Dinan 1 ; Jiang, Liqun 1 ; Wan, Lang 1 ; Li, Minhui 1 ; Jiang, 1 ;

作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Microbial Signals & Dis Co, Dept Plant Pathol, Guangzhou, Peoples R China

2.South China Agr Univ, Integrat Microbiol Res Ctr, Guangzhou, Peoples R China

3.Guangdong Acad Agr Sci, Rice Res Inst, Guangdong Prov Key Lab New Technol Rice Breeding, Guangzhou, Peoples R China

关键词： Peronophythora litchii; bZIP transcription factor; pathogenicity; peroxidase; laccase

期刊名称：MSPHERE （ 影响因子：4.389； 五年影响因子：4.988 ）

ISSN： 2379-5042

年卷期： 2020 年 5 卷 3 期

页码：

收录情况： SCI

摘要： Basic leucine zipper (bZIP) transcription factors are widespread in eukaryotes, including plants, animals, fungi, and oomycetes. However, the functions of bZIPs in oomycetes are rarely known. In this study, we identified a bZIP protein possessing a special bZIP-PAS structure in Peronophythora litchii, named PlBZP32. We found that PlBZP32 is upregulated in zoospores, in cysts, and during invasive hyphal growth. We studied the functions of PlBZP32 using the RNAi technique to suppress the expression of this gene. PlBZP32-silenced mutants were more sensitive to oxidative stress, showed a lower cyst germination rate, and produced more sporangia than the wild-type strain SHS3. The PlBZP32-silenced mutants were also less invasive on the host plant. Furthermore, we analyzed the activities of extracellular peroxidases and laccases and found that silencing PlBZP32 decreased the activities of P. litchii peroxidase and laccase. To our knowledge, this is the first report that the functions of a bZIP-PAS protein are associated with oxidative stress, asexual development, and pathogenicity in oomycetes. IMPORTANCE In this study, we utilized the RNAi technique to investigate the functions of PlBZP32, which possesses a basic leucine zipper (bZIP)-PAS structure, and provided insights into the contributions of bZIP transcription factors to oxidative stress, the production of sporangia, the germination of cysts, and the pathogenicity of Peronophythora litchii. This study also revealed the role of PlBZP32 in regulating the enzymatic activities of extracellular peroxidases and laccases in the plant-pathogenic oomycete.

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