江苏省农业科学院机构知识库

Paracellular Pathway-Mediated Mycoplasma hyopneumoniae Migration across Porcine Airway Epithelial Barrier under Air-Liquid Interface Conditions

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文献类型：外文期刊

作者： Wang, Haiyan ¹ ; Zhang, Zhenzhen ¹ ; Xie, Xing ¹ ; Liu, Beibei ¹ ; Wei, Yanna ¹ ; Gan, Yuan ¹ ; Yuan, Ting ¹ ; Ni, Bo ¹ ; Wa ¹ ;

作者机构： 1.Minist Agr, Key Lab Vet Biol Engn & Technol, Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Peoples R China

关键词： Mycoplasma hyopneumoniae; air-liquid interface; pig bronchial epithelial cells; barrier function; migration

期刊名称：INFECTION AND IMMUNITY （影响因子：3.441；五年影响因子：3.702 ）

ISSN： 0019-9567

年卷期： 2020 年 88 卷 10 期

页码：

收录情况： SCI

摘要： Mycoplasma hyopneumoniae is an important respiratory pathogen of pigs that causes persistent and secondary infections. However, the mechanisms by which this occurs are unclear. In this study, we established air-liquid interface culture systems for pig bronchial epithelial cells (ALI-PBECs) that were comparable to the conditions in the native bronchus in vivo. We used this ALI-PBECs model to study the infection and migration characteristics of M. hyopneumoniae in vitro. Based on the results, we confirmed that M. hyopneumoniae was able to adhere to ALI-PBECs and disrupt mucociliary function. Importantly, M. hyopneumoniae could migrate to the basolateral chamber through the paracellular route but not the transcellular pathway, and this was achieved by reversibly disrupting tight junctions (TJs) and increasing the permeability and damaging the integrity of the epithelial barrier. We examined the migration ability of M. hyopneumoniae using an ALI-PBECs model for the first time. The disruption of the epithelial barrier allowed M. hyopneumoniae to migrate to the basolateral chamber through the paracellular route, which may be related to immune evasion, extrapulmonary dissemination, and persistent infection of M. hyopneumoniae.

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Paracellular Pathway-Mediated Mycoplasma hyopneumoniae Migration across Porcine Airway Epithelial Barrier under Air-Liquid Interface Conditions

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