Paracellular Pathway-Mediated Mycoplasma hyopneumoniae Migration across Porcine Airway Epithelial Barrier under Air-Liquid Interface Conditions
文献类型： 外文期刊
作者： Wang, Haiyan 1 ; Zhang, Zhenzhen 1 ; Xie, Xing 1 ; Liu, Beibei 1 ; Wei, Yanna 1 ; Gan, Yuan 1 ; Yuan, Ting 1 ; Ni, Bo 1 ; Wa 1 ;
作者机构： 1.Minist Agr, Key Lab Vet Biol Engn & Technol, Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Peoples R China
关键词： Mycoplasma hyopneumoniae; air-liquid interface; pig bronchial epithelial cells; barrier function; migration
期刊名称：INFECTION AND IMMUNITY （ 影响因子：3.441； 五年影响因子：3.702 ）
ISSN： 0019-9567
年卷期： 2020 年 88 卷 10 期
页码：
收录情况： SCI
摘要： Mycoplasma hyopneumoniae is an important respiratory pathogen of pigs that causes persistent and secondary infections. However, the mechanisms by which this occurs are unclear. In this study, we established air-liquid interface culture systems for pig bronchial epithelial cells (ALI-PBECs) that were comparable to the conditions in the native bronchus in vivo. We used this ALI-PBECs model to study the infection and migration characteristics of M. hyopneumoniae in vitro. Based on the results, we confirmed that M. hyopneumoniae was able to adhere to ALI-PBECs and disrupt mucociliary function. Importantly, M. hyopneumoniae could migrate to the basolateral chamber through the paracellular route but not the transcellular pathway, and this was achieved by reversibly disrupting tight junctions (TJs) and increasing the permeability and damaging the integrity of the epithelial barrier. We examined the migration ability of M. hyopneumoniae using an ALI-PBECs model for the first time. The disruption of the epithelial barrier allowed M. hyopneumoniae to migrate to the basolateral chamber through the paracellular route, which may be related to immune evasion, extrapulmonary dissemination, and persistent infection of M. hyopneumoniae.
- 相关文献
作者其他论文 更多>>
-
Functional Genomics: From Soybean to Legume
作者：Zhou, Can;Wang, Haiyan;Qi, Zhaoming;Zhou, Can;Wang, Haiyan;Zhu, Xiaobin;Xia, Zhengjun;Li, Yuqiu;Zhang, Bo;Tadege, Million;Wu, Shihao
关键词：legume; T2T; genome; pangenome; functional genomics; soybean
-
Enhancing methane cracking through variable-frequency microwave heating: Improved energy efficiency and carbon nanotube formation
作者：Liu, Haolin;Liao, Wei;Wen, Mengyuan;Wang, Jia;Jiang, Jianchun;Ren, Jurong;Wang, Jia;Jiang, Jianchun;Wang, Shule;Meng, Xianzhi;Xiong, Lingming;Jin, Yanghao
关键词：Microwave frequency; Simulation; Methane cracking; Carbon nanotubes
-
Nano-adjuvant based on lipo-imiquimod self-assembly for enhanced foot-and-mouth disease virus vaccine immune responses via intradermal immunization
作者：Yin, Wenzhu;Ma, Fang;Deng, Bihua;Zhao, Yanhong;Zuo, Xiaoxin;Wang, Haiyan;Lu, Yu;Xu, Zeyu;Zhao, Yanhong;Lu, Yu;Yin, Wenzhu;Deng, Bihua
关键词：Imiquimod; Lipidation; Self-assembly; Nano-adjuvant; Intradermal immunity; Foot-and-mouth vaccine
-
Establishment and verification of GC-MS/MS method for four fluoroquinolones residues determination based on precolumn derivatization: Application to poultry eggs
作者：Ding, Hao;Gao, Pengfei;Xu, Junjie;Liu, Shuyu;Guo, Yawen;Cao, Yang;Hong, Yiwei;Zhang, Tao;Zhang, Genxi;Guo, Xiaodong;Dai, Guojun;Xie, Kaizhou;Ding, Hao;Xu, Junjie;Liu, Shuyu;Gao, Pengfei;Guo, Yawen;Cao, Yang;Hong, Yiwei;Zhang, Tao;Zhang, Genxi;Guo, Xiaodong;Dai, Guojun;Xie, Kaizhou;Xie, Xing;Pang, Maoda
关键词：Fluoroquinolones; Derivatization; Poultry eggs; GC-MS/MS
-
Analytical Method Established via Derivatization-Integrated Gas Chromatography-Tandem Mass Spectrometry for the Determination of Enrofloxacin and Ofloxacin Residues: A Focus on Eggs and Dairy Products
作者：Xu, Junjie;Cai, Jingjing;Cheng, Sihui;Cao, Yang;Hong, Yiwei;Zhang, Tao;Zhang, Genxi;Guo, Xiaodong;Dai, Guojun;Xie, Kaizhou;Xu, Junjie;Ding, Hao;Cai, Jingjing;Gao, Pengfei;Cheng, Sihui;Cao, Yang;Hong, Yiwei;Zhang, Tao;Zhang, Genxi;Guo, Xiaodong;Dai, Guojun;Xie, Kaizhou;Ding, Hao;Gao, Pengfei;Xie, Xing;Pang, Maoda
关键词：Eggs; Dairy products; Enrofloxacin; Ofloxacin; Derivatization; GC-MS/MS
-
An optimized SPE-UPLC-MS/MS method for simultaneous quantification of 11 tetracyclines in dairy products
作者：Gao, Pengfei;Ding, Hao;Xie, Kaizhou;Gao, Pengfei;Xie, Xing;Yin, Boxing;Yang, Renqin;Hong, Yiwei;Cao, Yang;Guo, Yawen;Xu, Junjie;Hong, Lu;Cai, Jingjing;Cheng, Sihui;Zhang, Genxi;Guo, Xiaodong;Xie, Kaizhou
关键词：tetracycline antibiotics; veterinary drug residues; milk safety; UPLC-MS/MS; multi-residue analysis
-
Construction of vitamin E-based oil-in-water nano-emulsion adjuvant enhances immunogenicity of inactivated pseudorabies virus vaccine
作者：Yin, Wenzhu;Chang, Chen;Zhu, Yalu;Ma, Fang;Wang, Haiyan;Lu, Yu;Tang, Bo;Deng, Bihua;Yin, Wenzhu;Zhu, Yalu;Lu, Yu;Deng, Bihua
关键词：Oil-in-water emulsion; Nano-adjuvant; Vitamin E; Pseudorabies virus; Immune response
