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Identification of contaminants as members of theBacillus pumilusgroup and analysis of contamination sources in the cultivation ofPleurotus geesteranusmushrooms

文献类型： 外文期刊

作者： Lu, Na 1 ; Yuan, Wei-Dong 1 ; Wu, You-Liang 2 ; Song, Ji-Ling 1 ; Ma, Lin 3 ; Wang, Wei-Ke 1 ; Yan, Jing 1 ; Chen, Zai-Mi 1 ;

作者机构： 1.Hangzhou Acad Agr Sci, Hangzhou 310024, Peoples R China

2.Jiangshan Inst Agr Sci, Jiangshan 324100, Peoples R China

3.Jiangsu Acad Agr Sci, Nanjing 210014, Jiangsu, Peoples R China

4.Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou 310058, Peoples R China

5.Zhejiang Agr Technol Extens Ctr, Hangzhou 310020, Peoples R China

关键词： Mushroom cultivation; Contaminants; B; pumilus; Contamination source; Primordial induction

期刊名称：JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.729； 五年影响因子：1.681 ）

ISSN： 1125-4653

年卷期：

页码：

收录情况： SCI

摘要： Oyster mushroom (Pleurotus geesteranusSinger) is grown widely in China withut problems, but occasionally severe bacterial contamination occurred in cultivation bags ofPleurotus geesteranusduring high summer temperatures in some companies in the Chunan county of Zhejiang province in 2018, belonging to the so-calledBacillus pumilusgroup by morphological characteristics, 16S rRNA, GyrB and aroE genes sequencing, fatty acid analysis and MALDI analysis. The inoculation tests confirmed thatBacillus pumilusstrains were contaminants causing mycelial disappearance ofP. geesteranus. The dual culture tests demonstrated that several strains ofB. pumiluswere able to inhibit mycelial growth ofP. geesteranus. In order to find the sources of contamination the, process of mushroom cultivation was investigated. A 41.0% contamination rate occurred in cultivation bags with sponge plugs, as opposed to a very low contamination rate (<4%) in cultivation bags with cotton plugs. It was also shown that a 13.0% bacterial isolation rate was obtained from autoclaved sponge pieces, as opposed to 2.0% or 0.0% from cotton pieces, respectively. Fifteen strains isolated all were identified by MALDI-Biotyper asBacillusspp., belonging to four species (Bacillus circulans,B. pumilus,B.cereusandB. halosaccharovorans). Dual culture tests showed thatB. circulans,B. cereusandB. halosaccharovoransstrains had not inhibitory effect on mycelial growth ofP. geesteranus. This study furthermore shows that sponge plugs containingB. pumiluscontaminants were the contamination source in cultivation bags ofP. geesteranus.

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