您好，欢迎访问贵州省农业科学院 机构知识库！
贵州省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Effects of simulated drought stress on carotenoid contents and expression of related genes in carrot taproots

文献类型： 外文期刊

作者： Zhang, Rong-Rong 1 ; Wang, Ya-Hui 1 ; Li, Tong 1 ; Tan, Guo-Fei 2 ; Tao, Jian-Ping 1 ; Su, Xiao-Jun 3 ; Xu, Zhi-Sheng; 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement, Minist Agr & Rural Affairs,Key Lab Biol & Germpla, Nanjing 210095, Peoples R China

2.Guizhou Acad Agr Sci, Inst Hort, Guiyang 55006, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210095, Peoples R China

关键词： Carrot; Taproot; Carotenoid; Gene expression; Drought stress

期刊名称：PROTOPLASMA （ 影响因子：3.356； 五年影响因子：3.298 ）

ISSN： 0033-183X

年卷期：

页码：

收录情况： SCI

摘要： Carotenoids are liposoluble pigments found in plant chromoplasts that are responsible for the yellow, orange, and red colors of carrot taproots. Drought is one of the main stress factors affecting carrot growth. Carotenoids play important roles in drought resistance in higher plants. In the present work, the carotenoid contents in three different-colored carrot cultivars, 'Kurodagosun' (orange), 'Benhongjinshi' (red), and 'Qitouhuang' (yellow), were determined by ultra-high-performance liquid chromatography (UPLC) after 15% polyethylene glycol (PEG) 6000 treatment. Real-time fluorescence quantitative PCR (RT-qPCR) was then used to determine the expression levels of carotenoid synthesis- and degradation-related genes. Increases in beta-carotene content in 'Qitouhuang' taproots under drought stress were found to be related to the expression levels of DcPSY2 and DcLCYB. Increases in lutein and decreases in alpha-carotene content in 'Qitouhuang' and 'Kurodagosun' under PEG treatment may be related to the expression levels of DcCYP97A3, DcCHXE, and DcCHXB1. The expression levels of DcNCED1 and DcNCED2 in the three cultivars significantly increased, thus suggesting that NCED genes could respond to drought stress. Analysis of the growth status and carotenoid contents of carrots under PEG treatment indicated that the orange cultivar 'Kurodagosun' has better adaptability to drought stress than the other cultivars and that beta-carotene and lutein may be involved in the stress resistance process of carrot.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：贵州省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台