Electrochemical immunosensor based on AuNPs/Zn/ Ni-ZIF-8-800@graphene for rapid detection of aflatoxin B1 in peanut oil
文献类型： 外文期刊
作者： Wang, Na 1 ; Liu, Qingquing 2 ; Hu, Xiaofei 4 ; Wang, Fan 2 ; Hu, Mei 1 ; Yu, Qiuying 2 ; Zhang, Gaiping 1 ;
作者机构： 1.Henan Agr Univ, Coll Vet Med, Zhengzhou 450002, Henan, Peoples R China
2.Henan Agr Univ, Coll Food Sci & Technol, Zhengzhou 450002, Henan, Peoples R China
3.Int Joint Res Ctr Anim Immunol, Zhengzhou 450002, Henan, Peoples R China
4.Henan Acad Agr Sci, Minist Agr, Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China
5.Key Lab Nutr & Hlth Food Zhengzhou, Zhengzhou 450002, Peoples R China
关键词： Electrochemical immunosensor; AuNPs; Zn; Ni-ZIF-8-800@graphene; Rapid detection; Aflatoxin B1; Peanut oil
期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.191； 五年影响因子：3.228 ）
ISSN： 0003-2697
年卷期： 2022 年 650 卷
页码：
收录情况： SCI
摘要： Peanut oil is a basic food raw material in life. However, aflatoxin contamination in peanut oil is considered to be one of the most serious food safety problems in the world. Based on AuNPs/Zn/Ni-ZIF-8-800@graphene composite, a simple, efficient and sensitive electrochemical immunosensor was developed to detect aflatoxin B1 (AFB1) in peanut oil. The bare glassy carbon electrode was modified by graphene, bimetallic organic framework material (Zn/Ni-ZIF-8-800), chitosan and gold nanoparticles. The electrochemical immunosensor was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FT-IR) and cyclic voltammetry (CV), and the electrochemical signal changes after antibody and AFB1 binding were investigated in detail. Under the optimal conditions, the linear range of the electrochemical immunosensor was 0.18-100 ng/mL, and the detection limit was 0.18 ng/mL. In addition, the prepared sensor has high selectivity and long-term stability, which lays a foundation for the simple, rapid and sensitive detection of AFB1 in peanut oil.
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