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The Broad Host Range Phage vB_CpeS_BG3P Is Able to Inhibit Clostridium perfringens Growth

文献类型： 外文期刊

作者： Huang, Sisi 1 ; Tian, Yuan 2 ; Wang, Yongjuan 4 ; Garcia, Pilar 5 ; Liu, Banhong 2 ; Lu, Rui 2 ; Wu, Liting 2 ; Bao, Hongduo 2 ; Pang, Maoda 2 ; Zhou, Yan 2 ; Wang, Ran 2 ; Zhang, Hui 2 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base MOST, Nanjing 210014, Peoples R China

3.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China

4.Jiangsu Agrianim Husb Vocat Coll, Taizhou 225300, Peoples R China

5.Dairy Res Inst Asturias IPLA CSIC, Villaviciosa 33300, Asturias, Spain

6.Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China

关键词： Clostridium perfringens; phage; antimicrobial agent; poultry industry

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2022 年 14 卷 4 期

页码：

收录情况： SCI

摘要： Clostridium perfringens is an important pathogen for both humans and animals, causing human foodborne disease and necrotic enteritis in poultry. In the present study, a C. perfringens-specific phage, vB_CpeS_BG3P (designated as BG3P hereafter), was isolated from chicken farm sewage. Both electron microscopy and phylogenetic analysis suggested that phage BG3P is a novel phage belonging to Siphoviridae family. Phage BG3P exhibited a broad host range against different C. perfringens isolates (90.63% of strains were infected). Sequencing of the complete genome revealed a linear double-stranded DNA (43,528 bp) with 28.65% GC content. After sequence analysis, 73 open reading frames (orfs) were predicted, of which only 13 were annotated with known functions. No tRNA and virulence encoding genes were detected. It should be noted that the protein of orf 15 has 97.92% homology to C. perfringens-specific chloramphenicol resistance protein, which has not been reported for any C. perfringens phage. Phylogenetic analysis of the ssDNA binding protein demonstrated that this phage is closely related to C. perfringens phages phiSM101 and phi3626. In considering future use as an antimicrobial agent, some biological characteristics were observed, such as a good pH (3-11) stability and moderate temperature tolerance (<60 degrees C). Moreover, bacteriophage BG3P showed a good antimicrobial effect against C. perfringens liquid cultures. Thus, phage treatment with MOI >= 100 completely inhibited bacterial growth compared to untreated cultures. Although phage BG3P shows good lytic efficiency and broad host range in vitro, future development and application may need to consider removal of the chloramphenicol-like resistance gene or exploring its lysin for future antibacterial applications.

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