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Determination of fourteen Sulfonamides residues in penaeus vannamei by high performance liquid chromatography coupled with post-column derivation

文献类型： 外文期刊

作者： Huang Dong-mei 1 ; Xu Jie 1 ; Shi Yong-fu 1 ; Huang Xuan-yun 1 ; Yu Huan 1 ; Feng Bing 1 ; Wang Yuan 1 ; Cai Youqiong 1 ;

作者机构： 1.Chinese Acad Fisheries Sci, East Sea Fisheries Res Insititute, Shanghai 200090, Peoples R China

关键词： Sulfonamides;high performance liquid chromatography;post-column derivation;penaeus vannamei;residuce detection

期刊名称：ADVANCES IN CHEMICAL ENGINEERING III, PTS 1-4

ISSN： 1022-6680

年卷期： 2013 年 781-784 卷

页码：

收录情况： SCI

摘要： The method was established to detect fourteen sulfonamides residuces in Penaeus vannamei by high performance liquid chromatography coupled with post-column derivation. Sulfonamides residues were extracted with ethyl acetate after adding sulfapyridine as internal standard. The extract was concentrated.The residues were transferred to hydrochloric acid solution. The solution was defatted with n-hexane. The compounds were detected by HPLC with fluorescence detector.The standard addition method was used. The calibration curves were linear. The recoveries ranged from 77.8% to 103.6%. The relative standard deviations were all below 9.1%. Quantitative detection limits of fourteen sulfanomides ranged from 1.0 mu g/kg to 5.0 mu g/kg. Results indicated that the method was easier, faster and more accurate.

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