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Proteomic Analysis Associated with the Immune Response in Hemocytes of Portunus trituberculatus Challenged with Vibrio parahaemolyticus

文献类型： 外文期刊

作者： Gao, Baoquan 1 ; Ren, Xianyun 1 ; Lv, Jianjian 1 ; Meng, Xianliang 1 ; Liu, Ping 1 ; Li, Jian 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Peoples R China

关键词： Portunus trituberculatus; Vibrio parahaemolyticus; molecular mechanism; comparative proteome analysis; iTRAQ

期刊名称：FISHES （ 影响因子：3.17； 五年影响因子：3.098 ）

ISSN：

年卷期： 2022 年 7 卷 5 期

页码：

收录情况： SCI

摘要： Vibrio parahaemolyticus belongs to an expanding group of aquatic pathogens that are widely distributed in aquatic environments. This species is a lethal pathogen for a number of economically important marine crabs. However, studies exploring host-vibrio interactions between V. parahaemolyticus and crabs are scarce, and therefore, the underlying molecular mechanisms are unclear. Herein, we performed a comprehensive proteomic analysis to investigate the immune response of Portunus trituberculatus hemocytes to V. parahaemolyticus infection. A total of 4433 proteins were identified using isobaric tags for relative and absolute quantitation (iTRAQ), and 526 differentially expressed proteins (DEPs) were subjected to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, with six DEPs further subjected to quantitative real-time PCR. Several identified DEPs were found to be mainly involved in the immune defense of the crustacean, such as a hemocyanin subunit, C-type lectin, alpha-2-macroglobulin, Cu/Zn-superoxide dismutase, and heat shock protein 70, playing a key role in the response to V. parahaemolyticus infection. Moreover, many immune-related KEGG pathways were markedly altered, such as cell adhesion molecules, complement and coagulation cascades, and phagosomes. Our results provide insights into how V. parahaemolyticus overcomes the innate immunity of P. trituberculatus to induce pathological alterations in affected tissues. We report the first iTRAQ-based proteomic analysis and highlight the key pathways and proteins involved in the host-vibrio interactions between P. trituberculatus and V. parahaemolyticus. These findings should enhance our understanding of the molecular mechanisms underlying such interactions.

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