Beneficial Effect of Selenium Doped Carbon Quantum Dots Supplementation on the in vitro Development Competence of Ovine Oocytes
文献类型： 外文期刊
作者： Wang, Mengqi 1 ; Ren, Jingyu 1 ; Liu, Zhanpeng 2 ; Li, Shubin 3 ; Su, Liya 1 ; Wang, Biao 4 ; Han, Daoning 1 ; Liu, Gang 1 ;
作者机构： 1.Affiliated Hosp Inner Mongolia Med Univ, Clin Med Res Ctr, Key Lab Med Cell Biol, Hohhot, Inner Mongolia, Peoples R China
2.Inner Mongolia Univ, Coll Life Sci, Hohhot, Inner Mongolia, Peoples R China
3.Inner Mongolia Peoples Hosp, Dept Geriatr Med Ctr, Hohhot, Inner Mongolia, Peoples R China
4.Inner Mongolia Acad Agr & Anim Husb Sci, Anim Husb Inst, Hohhot, Inner Mongolia, Peoples R China
关键词： Se; CDs; mitochondrial activity; reactive oxygen species production; ovine oocytes and transcript profiles
期刊名称：INTERNATIONAL JOURNAL OF NANOMEDICINE （ 影响因子：7.033； 五年影响因子：7.419 ）
ISSN： 1178-2013
年卷期： 2022 年 17 卷
页码：
收录情况： SCI
摘要： Background: After the synthesis of selenium doped carbon quantum dots (Se/CDs) via a step-by-step hydrothermal synthesis method with diphenyl diselenide (DPDSe) as precursor, the beneficial effects of Se/CDs' supplementation on the in vitro development competence of ovine oocytes were firstly investigated in this study by the assay of maturation rate, cortical granules' (CGs) dynamics, mitochondrial activity, reactive oxygen species (ROS) production, epigenetic modification, transcript profile, and embryonic devel-opment competence.Results: The results showed that the Se/CDs' supplementation during the in vitro maturation (IVM) process not only enhanced the maturation rate, CGs' dynamics, mitochondrial activity and embryonic developmental competence of ovine oocytes, but remarkably decreased the ROS production level of ovine oocytes. In addition, the expression levels of H3K9me3 and H3K27me3 in the ovine oocytes were significantly up-regulated after the Se/CDs' supplementation, in consistent with the expression levels of 5mC and 5hmC. Moreover, 2994 up-regulated differentially expressed genes (DEGs) and 846 repressed DEGs were found in the oocytes after the Se/ CDs' supplementation. According to the analyses of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), these DEGs induced by the Se/CDs' supplementation were positively related to the progesterone mediated oocyte maturation and mitochondrial functions. And these remarkably up-regulated expression levels of DEGs related to oocyte maturation, mitochondrial function, and epigenetic modification induced by the Se/CDs' supplementation further confirmed the beneficial effect of Se/CDs' supplementation on the in vitro development competence of ovine oocytes.Conclusion: The Se/CDs prepared in our study significantly promoted the in vitro development competence of ovine oocytes, benefiting the extended research about the potential applications of Se/CDs in mammalian breeding technologies.
- 相关文献
作者其他论文 更多>>
-
Heat Tolerance Differences Between Hu Sheep and Hu Crossbred Sheep in Microbial Community Structure and Metabolism
作者：Yuan, Jing-Da;Wang, Li-Wei;Fu, Shao-Yin;E, Ri-Ge-Li-Tu;Ren, Xiao-Qi;Sun, Hua;Liu, Fang;Wang, Biao;An, Jiang-Hong;Zhao, Meng-Ran;He, Jiang-Feng;He, Xiao-Long;Yuan, Jing-Da;E, Ri-Ge-Li-Tu;Ren, Xiao-Qi;He, Jiang-Feng;He, Xiao-Long
关键词：heat stress; meat sheep; thermotolerance; metabolome; gut microbiota
-
Transcriptome Analysis Reveals the Molecular Mechanisms by Which ADAMTS1 Influences the Proliferation of Ovarian Granulosa Cells in Sheep
作者：Li, Rongqing;Zhang, Wenjia;Gao, Yuanshuai;Xie, Zhiqiang;Fang, Qinyuan;Gao, Min;Wang, Zheng;Zhang, Teng;Liu, Yongbin;He, Jiangfeng;Liu, Fang;Wang, Biao;El-Sherbiny, Mohamed;Liu, Yongbin
关键词：ADAMTS1; PSAT1; granulosa cells; proliferation; apoptosis; follicle; follicular development; follicular atresia; oocyte; SLC6A9
-
A near complete genome assembly of the East Friesian sheep genome
作者：You, Xiaoxiao;Fang, Qinyuan;Zhang, Teng;Zhou, Yang;Wang, Zheng;Sun, Tianhao;Zhou, Huanmin;Dai, Yanfeng;Liu, Yongbin;Chen, Chunhai;Yang, Xukui;Jian, Jianbo;Cao, Junwei;Wang, Shenyuan;Zhou, Huanmin;Fu, Shaoyin;He, Xiaolong;He, Jiangfeng;Wang, Biao;Wang, Liwei;Te, Rigele;Liu, Yongbin
关键词：
-
Effect of Gentianella acuta (Michx.) Hulten against the arsenic-induced development hindrance of mouse oocytes
作者：Wang, Chunyu;Wei, Ying;Liu, Gang;Wang, Chunyu;Wang, Biao;Li, Shubin;Ren, Jingyu;Dai, Yanfeng
关键词：Gentianella acuta; Arsenic; Oocytes; Redox regulation; H3K27me3
-
Differential immunological responses in lamb rumen and colon to alfalfa hay and wheat straw in a concentrate-rich diet: insights into microbe-host interactions
作者：Gebeyew, Kefyalew;Mi, Hui;Liu, Yong;Tan, Zhiliang;He, Zhixiong;Gebeyew, Kefyalew;Mi, Hui;Liu, Yong;Tan, Zhiliang;He, Zhixiong;Liu, Yongbin;Wang, Biao;Feyera, Teka
关键词：concentrate-rich diet; interaction; lamb; microbiome; transcriptome
-
Selection signature analysis reveals genes associated with tail phenotype in sheep
作者：Qi, Yunxia;Yang, Chaoyun;He, Xiaolong;Wang, Biao;Da, Lai;Fu, Shaoyin;Liu, Yongbin;Liu, Bin;Zhang, Wenguang;Liu, Yongbin
关键词：sheep; tail type; selection signature analysis; F-ST; pi-ratio; XP-EHH
-
Blood Metabolites and Faecal Microbial Communities in Nonpregnant and Early Gestation Ewes in Highly Cold Areas
作者：Wu, Zhiwu;Gebeyew, Kefyalew;Tang, Shaoxun;Han, Xuefeng;He, Zhixiong;Tan, Zhiliang;Wu, Zhiwu;Gebeyew, Kefyalew;Han, Xuefeng;He, Zhixiong;Tan, Zhiliang;Yang, Yanyan;Wang, Biao
关键词：early gestation; pregnancy; metabolite; faecal microbiota; biomarker
