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Leonurine improves bovine oocyte maturation and subsequent embryonic development by reducing oxidative stress and improving mitochondrial function

文献类型： 外文期刊

作者： Zheng, Lv 1 ; Luo, Yuwen 1 ; Zhou, Dan 1 ; Liu, Hongyu 1 ; Zhou, Guizhen 1 ; Meng, Lin 2 ; Hou, Yunpeng 2 ; Liu, Changbin 3 ; Li, Jun 4 ; Fu, Xiangwei 1 ;

作者机构： 1.Coll Anim Sci, Key Lab Anim Genet, Beijing Key Lab Anim Genet Improvement,Natl Engn L, Breeding & Reprod Minist Agr & Rural Affairs, Beijing 100193, Peoples R China

2.China Agr Univ, Coll Biol Sci, State Key Labs Agrobiotechnol, Beijing 100193, Peoples R China

3.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Breed, Inst Anim Husb & Vet Sci, Shihezi 832000, Peoples R China

4.First Hosp Hebei Med Univ, Reprod Med Ctr, Dept Reprod Med, Shijiazhuang 050031, Hebei, Peoples R China

关键词： Bovine; IVP; Leonurine (LEO); Mitochondria; Antioxidant

期刊名称：THERIOGENOLOGY （ 影响因子：2.8； 五年影响因子：2.6 ）

ISSN： 0093-691X

年卷期： 2023 年 199 卷

页码：

收录情况： SCI

摘要： It is acknowledged that excessive reactive oxygen species (ROS) level attributes greatly to the compro-mised developmental potential of oocytes matured in vitro. Although agents were applied to alleviate ROS levels, results were varied because of the distinct antioxidative activity and cell toxicity. Leonurine (LEO), extracted from the natural Chinese herb motherwort, is considered to be a potent free radical scavenger. Yet, it is undetermined whether LEO is benefit for oocyte development during in vitro maturation (IVM). In the present study, the effect of LEO on the quality of bovine oocyte as well as the underlying mechanism was investigated. We found that maturation rate (P < 0.01), subsequent blasto-cyst formation rate (P < 0.05), and the total blastocyst cell number (P < 0.05) after parthenogenetic activation were significantly increased in the group treated with 20 mM LEO. Moreover, a dramatic decline in ROS (P < 0.01), decreased lipid content (P < 0.01), elevated MMP level (P < 0.05), increased ATP content (P < 0.05), and reduced mitochondrial temperature (P < 0.01) were observed in oocytes treated with LEO. Furthermore, the expression level of anti-apoptotic protein BCL2 was significantly higher in LEO treated oocytes (P < 0.01), and the ratio of BAX/BCL2 was obvious decreased (P < 0.01). Finally, we found that LC3B intensity was significantly reduced (P < 0.05) while the rate of EdU positive nuclei was markedly increased (P < 0.05) in embryos derived from LEO-treated oocytes. Our results demonstrate that LEO exhibits a potent protective role in the acquisition of oocyte development capacity against oxidative stress during IVM, and provides a new solution for optimizing the in vitro culture system of bovine embryos.(c) 2023 Published by Elsevier Inc.

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