文献类型： 外文期刊

作者： Yang, Mengmeng ¹ ; Yang, Fei ¹ ; Guo, Yanan ² ; Liu, Fan ¹ ; Li, Yong ⁴ ; Qi, Yanrong ⁵ ; Guo, Lei ¹ ; He, Shenghu ¹ ;

作者机构： 1.Ningxia Univ, Coll Anim Sci & Technol, Yinchuan, Ningxia, Peoples R China

2.Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Yinchuan, Ningxia, Peoples R China

3.Ningxia Med Univ, Sch Basic Med, Yinchuan, Ningxia, Peoples R China

4.Ningxia Polytech, Coll Life Sci & Technol, Yinchuan, Ningxia, Peoples R China

5.Agr & Rural Bur Helan Cty, Yinchuan, Ningxia, Peoples R China

关键词： Dang-Shen-Yu-Xing decoction; Mycoplasma bovis pneumonia; network pharmacology; IL6; IL10

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：2.9； 五年影响因子：3.3 ）

ISSN：

年卷期： 2024 年 11 卷

页码：

收录情况： SCI

摘要： Mycoplasma bovis pneumonia is a highly contagious respiratory infection caused by Mycoplasma bovis. It is particularly prevalent in calves, posing a significant threat to animal health and leading to substantial economic losses. Dang-Shen-Yu-Xing decoction is often used to treat this condition in veterinary clinics. It exhibits robust anti-inflammatory effects and can alleviate pulmonary fibrosis. However, its mechanism of action remains unclear. Therefore, this study aimed to preliminarily explore the molecular mechanism of Dang-Shen-Yu-Xing decoction for treating mycoplasma pneumonia in calves through a combination of network pharmacology, molecular docking, molecular dynamics simulation methods, and experimental validation. The active components and related targets of Dang-Shen-Yu-Xing decoction were extracted from several public databases. Additionally, complex interactions between drugs and targets were explored through network topology, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. Subsequently, the binding affinity of drug to disease-related targets was verified through molecular docking and molecular dynamics simulation. Finally, the pharmacodynamics were verified via animal experiments. The primary network topology analysis revealed two core targets and 10 key active components of Dang-Shen-Yu-Xing decoction against Mycoplasma bovis pneumonia. Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that the mechanism of Dang-Shen-Yu-Xing decoction for treating mycoplasma bovis pneumonia involved multiple signaling pathways, with the main pathways including PI3K-Akt and IL17 signaling pathways. Moreover, molecular docking predicted the binding affinity and conformation of the core targets of Dang-Shen-Yu-Xing decoction, IL6, and IL10, with the associated main active ingredients. The results showed a strong binding of the active ingredients to the hub target. Further, molecular docking dynamics simulation revealed three key active components of IL10 induced by Dang-Shen-Yu-Xing decoction against Mycoplasma bovis pneumonia. Finally, animal experiments confirmed Dang-Shen-Yu-Xing decoction pharmacodynamics, suggesting that it holds potential as an alternative therapy for treating mycoplasma bovis pneumonia.