文献类型： 外文期刊

作者： Zhang, Feng ¹ ; Yang, Chenkun ³ ; Guo, Hao ³ ; Li, Yufei ³ ; Shen, Shuangqian ³ ; Zhou, Qianqian ² ; Li, Chun ³ ; Wang, Chao ³ ; Zhai, Ting ¹ ; Qu, Lianghuan ² ; Zhang, Cheng ¹ ; Liu, Xianqing ³ ; Luo, Jie ³ ; Chen, Wei ² ; Wang, Shouchuang ³ ; Yang, Jun ³ ; Yu, Cui ¹ ; Liu, Yanyan ² ;

作者机构： 1.Hubei Acad Agr Sci, Ind Crops Inst, Wuhan 430064, Hubei, Peoples R China

2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China

3.Hainan Univ, Sanya Inst Breeding & Multiplicat, Sch Breeding & Multiplicat, Sanya 572025, Hainan, Peoples R China

4.Yazhouwan Natl Lab, Sanya 572025, Hainan, Peoples R China

5.Hubei Hongshan Lab, Wuhan 430070, Hubei, Peoples R China

关键词： UV-B stress; Metabolome; Transcriptome; mGWAS; Genetic basis; Natural variation; Rice

期刊名称：GENOME BIOLOGY （ 影响因子：9.4； 五年影响因子：16.3 ）

ISSN： 1474-760X

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundUV-B, an important environmental factor, has been shown to affect the yield and quality of rice (Oryza sativa) worldwide. However, the molecular mechanisms underlying the response to UV-B stress remain elusive in rice.ResultsWe perform comprehensive metabolic profiling of leaves from 160 diverse rice accessions under UV-B and normal light conditions using a widely targeted metabolomics approach. Our results reveal substantial differences in metabolite accumulation between the two major rice subspecies indica and japonica, especially after UV-B treatment, implying the possible role and mechanism of metabolome changes in subspecies differentiation and the stress response. We next conduct a transcriptome analysis from four representative rice varieties under UV-B stress, revealing genes from amino acid and flavonoid pathways involved in the UV-B response. We further perform a metabolite-based genome-wide association study (mGWAS), which reveals 3307 distinct loci under UV-B stress. Identification and functional validation of candidate genes show that OsMYB44 regulates tryptamine accumulation to mediate UV-B tolerance, while OsUVR8 interacts with OsMYB110 to promote flavonoid accumulation and UV-B tolerance in a coordinated manner. Additionally, haplotype analysis suggests that natural variation of OsUVR8groupA contributes to UV-B resistance in rice.ConclusionsOur study reveals the complex biochemical and genetic foundations that govern the metabolite dynamics underlying the response, tolerance, and adaptive strategies of rice to UV-B stress. These findings provide new insights into the biochemical and genetic basis of the metabolome underlying the crop response, tolerance, and adaptation to UV-B stress.