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Preliminary study of the intranuclear function of Sma and Mad related protein 5 gene in Litopenaeus vannamei

文献类型： 外文期刊

作者： Li, Bin -Bin 1 ; Fan, Jin-Quan 1 ; Hong, Qian-Ming 1 ; Yang, Xin-Jun 1 ; Yan, Ze-Yu 1 ; Huang, Wen 3 ; Chen, Yi-Hong 1 ;

作者机构： 1.South China Normal Univ, Inst Modern Aquaculture Sci & Engn IMASE, Coll Life Sci, Key Lab Hlth & Safe Aquaculture, Guangzhou 510631, Peoples R China

2.Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China

3.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Peoples R China

关键词： Translocation in renal carcinoma on; chromosome 8; Sma and mad related protein 5; Litopenaeus vannamei; Unfolded protein response; ER -Stress

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：2.9； 五年影响因子：3.1 ）

ISSN： 0145-305X

年卷期： 2023 年 139 卷

页码：

收录情况： SCI

摘要： Litopenaeus vannamei Smad5 (LvSmad5) in cytoplasm has been proved to be involved in environmental stress response. As LvSmad5 could also locate in nucleus under specific stress, it was conjectured that LvSmad5 might participate in environmental stress response. While, the experimental evidence is still lacking. In this study, cytosolic LvSmad5 mutant or nuclear LvSmad5 mutant was expressed in Drosophila S2 cells, and then transcriptomic analysis of mentioned cells was performed using Illumina HiSeq based RNA-Seq, to reveal the function of LvSmad5 in nucleus. By comparing the two groups of cDNA libraries from S2 cells with cytosolic or nucleus LvSmad5 mutant, 86 differentially expressed genes as well as 765 differentially expressed transcripts were found. It was revealed that genes in the ER-stress response pathway, such as unfolded protein response and ERassociated degradation (ERAD) were enriched. Additionally, some kinds of metabolic reprogramming occurred in S2 cells with over-expressing nuclear LvSmad5, for significant changes in the expression of some metabolismrelated genes. To test our infer that nuclear LvSmad5 was engaged in environmental stress response, homologous gene of Drosophila translocation in renal carcinoma on chromosome 8 in L.vannamei (LvTRC8) was chosen for further investigation. And studies about LvTRC8, a member of ERAD showed that it was induced by ER-stress or heat shock treatment. Suppressed the expression of LvTRC8 increased the cumulative mortality of shrimp upon stress. In some degree, these results support our speculation that nuclear LvSmad5 are involved in the environmental stress response of L. vannamei in fact.

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