文献类型： 外文期刊

作者： Mo, Ziran ¹ ; Quan, Jingwei ¹ ; Xu, Bin ³ ; Yu, Huixia ⁷ ; Li, Junyan ⁶ ; Luo, Xiaoping ⁶ ; Wuyun, Qimuge ¹ ; Li, Jian ¹ ; Yang, Wenbin ¹ ; Hu, Wei ¹ ;

作者机构： 1.Inner Mongolia Univ, Coll Life Sci, Hohhot 010070, Peoples R China

2.Inner Mongolia Univ, Inst Biomed Sci, Hohhot 10070, Peoples R China

3.Fudan Univ, Chinese Ctr Dis Control & Prevent, Minist Hlth, Natl Inst Parasit Dis,WHO Collaborating Ctr Trop D, Shanghai 200025, Peoples R China

4.Guangxi Univ Tradit Chinese Med, Basic Med Coll, Nanning 530023, Guangxi, Peoples R China

5.Fudan Univ, Huashan Hosp, Minist Educ, State Key Lab Genet Engn,Sch Life Sci,Dept Infect, Shanghai 200438, Peoples R China

6.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010031, Peoples R China

7.Chifeng Ctr Dis Control & Prevent, Chifeng 024000, Peoples R China

关键词： Intestinal protozoan; Epidemiology; Genotype; Haplotype; One health

期刊名称：ONE HEALTH （ 影响因子：4.5； 五年影响因子：4.7 ）

ISSN：

年卷期： 2024 年 19 卷

页码：

收录情况： SCI

摘要： Cryptosporidium, Giardia duodenalis, and Enterocytozoon bieneusi are widespread zoonotic pathogens causing gastrointestinal diseases in humans and various animal species. Inner Mongolia, a major beef production region in China, there is a notable absence of comprehensive research on intestinal parasitism. Thus, timely and comprehensive diagnosis is essential to mitigate disease spread and minimize economic losses in the livestock industry. In this study, we collected fecal samples from cattle and humans, as well as soil and water samples, and all samples were tested for pathogenic intestinal protozoa at the Simmental cattle ranch in Wengniute, Chifeng City, Inner Mongolia. Among the 393 samples tested, 76/371 (20.5 %) cattle, 6/11 (54.5 %) ranch workers, 1/7 (14.3 %) water, and 2/4 (50 %) soil samples were positive. Factors affecting the infection rate of intestinal protozoa were examined. Results showed that the infection rate was higher in June than in January, higher in calves than in adults, and higher in diarrheal calves than in healthy calves. Additionally, the infection rate of intestinal protozoa was higher in pathogen-contaminated water source sheds than in uncontaminated sheds. Genetic and evolutionary analyses revealed that the prevalent E. bieneusi subtypes are predominantly J, I, and BEB4, while the G. duodenalis subtypes are assemblages B and E. The Cryptosporidium species identified were C. bovis, C. andersoni, C. parvum, C. ryanae, and C. suis, with C. parvum being a notable zoonotic pathogen. The pathogen sequences from humans, cattle, water, and soil showed 99-100 % similarity, suggesting possible transmission or contamination between animals and the environment. This study contributes to the One Health approach by addressing the gap in research on intestinal protozoa in Inner Mongolia. It provides important data for other ranches in the region to understand the prevalence of such pathogens and develop effective control measures. Using the concept of One Health to analyze the spatiotemporal distribution of intestinal protozoa in pastures is of great significance for maintaining public health.