文献类型： 外文期刊

作者： Worlanyo, Hanu Godfried ¹ ; Jiang, Sufei ¹ ; Yu, Yebin ⁴ ; Liu, Bo ¹ ; Zhou, Qunlan ¹ ; Sun, Cunxin ¹ ; Miao, Linghong ¹ ; Lin, Yan ¹ ; Zheng, Xiaochuan ¹ ; Saidyleigh, Momodou ¹ ; Lv, Bin ² ; Munganga, Brian Pelekelo ² ;

作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resources, Minist Agr & Rural Affairs,Key Lab Integrated Rice, Wuxi 214081, Peoples R China

2.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China

3.Minist Fisheries & Aquaculture Dev, Fisheries Commiss, POB 630, Accra, Ghana

4.Yancheng Inst Technol, Coll Marine Sci & Bioengn, Yancheng, Peoples R China

5.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, 9 Shanshui East Rd, Wuxi 214081, Peoples R China

关键词： Macrobrachium nipponense; Threonine; Growth performance; Antioxidant function; Immune response

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.622； 五年影响因子：4.799 ）

ISSN： 1050-4648

年卷期： 2022 年 128 卷

页码：

收录情况： SCI

摘要： A 70-day feeding trial was conducted to ascertain the effects of threonine on immune response of juvenile ori-ental river prawn (Macrobrachium nipponense). Six isonitrogen and isolipidic feeds were formulated according to levels of dietary threonine (0.35%, 0.79%, 1.18%, 1.67%, 2.08% and 2.48% respectively). The juvenile prawns were divided into six groups with four replicates, and stocked into 24 tanks with 50 prawns per tank (initial weight 0.20 +/- 0.02 g). The results showed a significant increasing trend of final body weight, specific growth rate, protein efficiency ratio, and weight gain rate when threonine levels increased to 1.67% (P < 0.05). However, feed intake, feed conversion ratio, and whole-body lipid composition significantly decreased as threonine levels in the feed increased up to 1.67% (P < 0.05). Moreover, haemolymph N-urea content was significantly lowest at 1.67% threonine level (P < 0.05), whereas glucose was highest at 0.79% followed by 1.67% of threonine levels in the feeds. Aspartate aminotransferase (AST) enzyme activities were significantly decreased by an imbalance (except 1.67%) of threonine in the feed (P < 0.05). Activities of Alanine amino-transferase (ALT) and albumen (ALB) were not significantly affected by threonine in the feed (P > 0.05). Excessive dietary threonine level (2.48%) significantly activated haemolymph catalase (CAT) activity (P < 0.05), whereas malondialdehyde (MDA) content was significantly affected by deficient (0.35% and 0.79%) dietary threonine levels (P < 0.05). Inducible nitric oxide synthase (iNOS) activity and haemolymph complement component 4 (C4) content were significantly decreased by deficient levels of threonine in the feed (P < 0.05). Excess threonine concentration significantly down-regulated Toll, Dorsal, Relish, and heat shock protein 60 (Hsp60) gene expressions in the hepatopancreas of M. nipponense (P < 0.05), while all genes were significantly up-regulated by the optimal (1.67%) threonine level (P < 0.05). The threonine level at which maximum specific growth rate of M. nipponense occurred was estimated by second degree polynomial regression analysis as 1.65% of threonine level, equivalent to 4.44% dry weight bases of protein in the feed.