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Induction of Mitogynogenetic Diploids and Identification of WW Super-female Using Sex-Specific SSR Markers in Half-Smooth Tongue Sole (Cynoglossus semilaevis)

文献类型： 外文期刊

作者： Chen, Song-Lin 1 ; Ji, Xiang-Shan 1 ; Shao, Chang-Wei 1 ; Li, Wen-Long 1 ; Yang, Jing-Feng 1 ; Liang, Zuo 1 ; Liao, Xi 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Shandong Agr Univ, Coll Anim Sci & Technol, Tai An 271018, Shandong, Peoples R China

关键词： Half-smooth tongue sole;Cynoglossus semilaevis;Mitogynogenesis;Pressure shock;Sex-linked SSR markers;WW super-female

期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：3.619； 五年影响因子：3.322 ）

ISSN： 1436-2228

年卷期： 2012 年 14 卷 1 期

页码：

收录情况： SCI

摘要： The half-smooth tongue sole (Cynoglossus semilaevis) is an important cultured marine fish as well as a promising model fish for the study of sex determination mechanisms. In the present study, a protocol for artificial induction of mitogynogenesis by hydrostatic pressure using heterologous sperm was developed in half-smooth tongue sole in order to assess homozygosity of gynogens and to identify WW super-female. The optimal initiation time for pressure shock of mitogynogenetic embryos was determined to be 21.5 min after insemination when water temperature is at 22-23A degrees C, while the optimal pressure and treatment duration were determined to be 70 MPa for 4 min. About 1,500 mitogynogenetic diploid larvae were obtained. Ten tongue sole microsatellite markers were used for homozygosity analysis of 24 mitogynogenetic larvae. Among the 24 larvae, the percentage of homozygosity ranged from 73.91% to 87.50% with an average homozygosity of 80.54%. Sex-specific simple sequence repeat (SSR) markers, CseF-SSR1, were isolated and used for identifying WW super-female mitogynogens in the tongue sole. The amplification of genomic DNA using the sex-specific SSR marker produced one DNA band of 206 bp in ZZ males, two DNA bands of 206 and 218 bp in ZW females, and one DNA band of 218 bp in WW super-females. Four WW "super-female" gynogens were observed in 39 mitogynogenetic diploids, indicating a ZW sex determination mechanism in the tongue sole. Thus, a protocol for the induction of artificial mitogynogenesis has been developed for the first time in half-smooth tongue sole, and the WW super-female diploids were identified in the mitogynogens by sex-specific SSR markers. These findings lay the foundation and provide important tool for the elaboration of sex determination mechanism, generation of WW super-females, and development of clone line and breeding of all-female stock in the half-smooth tongue sole.

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[1]Growth differences and differential expression analysis of pituitary adenylate cyclase activating polypeptide (PACAP) and growth hormone-releasing hormone (GHRH) between the sexes in half-smooth tongue sole Cynoglossus semilaevis. Ji, Xiang-Shan,Chen, Song-Lin,Xu, Tian-Jun,Yang, Jing-Feng,Tian, Yong-Sheng,Ji, Xiang-Shan,Jiang, Yun-Liang. 2011

[2]Molecular cloning, characterization and expression analysis of Sox9a and Foxl2 genes in half-smooth tongue sole (Cynoglossussemilaevis). Dong Xiaoli,Chen Songlin,Shao Changwei,Dong Xiaoli,Ji Xiangshan. 2011

[3]A first generation BAC-based physical map of the half-smooth tongue sole (Cynoglossus semilaevis) genome. Zhang, Junjie,Shao, Changwei,Zhang, Liyan,Liu, Kun,Gao, Fengtao,Dong, Zhongdian,Chen, Songlin,Zhang, Junjie,Zhang, Junjie,Xu, Peng. 2014

[4]Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis. Liu Yunguo,Liu Yunguo,Li Junfeng,Ye Naihao. 2011

[5]Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis. Zhang, Bo,Wang, Xianli,Sha, Zhenxia,Yang, Changgeng,Liu, Shanshan,Wang, Na,Chen, Song-Lin,Zhang, Bo,Liu, Shanshan.

[6]Molecular and phenotypic characterization of Vibrio aestuarianus, a pathogen of the cultured tongue sole, Cynoglossus semilaevis Gunther. Zhang, X-J,Qin, G-M,Yan, B-L,Liang, L-G,Bing, X-W. 2011

[7]Comparison of chromosome preparation methods for the different developmental stages of the half-smooth tongue sole, Cynoglossus semilaevis. Shao, Chang-Wei,Wu, Peng-Fei,Wang, Xian-Li,Tian, Yong-Sheng,Chen, Song-Lin. 2010

[8]Construction of a microsatellite-based genetic linkage map for half-smooth tongue sole Cynoglossus semilaevis. Song, Wentao,Miao, Guidong,Zhao, Yongwei,Niu, Yuze,Pang, Renyi,Liao, Xiaolin,Shao, Changwei,Chen, Songlin,Song, Wentao. 2013

[9]Isolation of Female-Specific AFLP Markers and Molecular Identification of Genetic Sex in Half-Smooth Tongue Sole (Cynoglossus semilaevis). Chen, Song-Lin,Li, Jing,Deng, Si-Ping,Tian, Yong-Sheng,Wang, Qing-Yin,Zhuang, Zhi-Meng,Sha, Zhen-Xia,Xu, Jian-Yong.

[10]Construction of Two BAC Libraries from Half-Smooth Tongue Sole Cynoglossus semilaevis and Identification of Clones Containing Candidate Sex-Determination Genes. Shao, Chang-Wei,Chen, Song-Lin,Xu, Jian-Yong,Sha, Zhen-Xia,Dong, Xiao-Li,Shao, Chang-Wei,Scheuring, Chantel F.,Zhang, Hong-Bin,Shao, Chang-Wei,Scheuring, Chantel F.,Zhang, Hong-Bin.

[11]Establishment and characterization of a new fish cell line from head kidney of half-smooth tongue sole (Cynoglossus semilaevis). Zheng, Yuan,Wang, Na,Xie, Ming-Shu,Sha, Zhen-Xia,Chen, Song-Lin,Zheng, Yuan,Xie, Ming-Shu.

[12]Molecular cloning, genomic structure and expression analysis of major histocompatibility complex class I alpha gene of half-smooth tongue sole (Cynoglossus semilaevis). Chen, Song-lin,Xu, Tian-jun. 2011

[13]Genomic structure, polymorphism and expression analysis of growth hormone-releasing hormone and pituitary adenylate cyclase activating polypeptide genes in the half-smooth tongue sole (Cynoglossus semilaevis). Ma, Q.,Liu, S. F.,Zhuang, Z. M.,Lin, L.,Sun, Z. Z.,Liu, C. L.,Tang, Q. S.,Ma, Q.,Lin, L.,Su, Y. Q.. 2011

[14]Characterization of the cyp19a1a gene from a BAC sequence in half-smooth tongue sole (Cynoglossus semilaevis) and analysis of its conservation among teleosts. Shao Changwei,Shao Changwei,Liu Shanshan,Liu Changlin,Chen Songlin,Liu Geng. 2013

[15]Unexpected variations Of beta(2)-microglobulin gene in the half-smooth tongue sole. Xu, Tian-jun,Sha, Zhen-xia,Chen, Song-lin,Xu, Tian-jun. 2010

[16]Molecular characterization and expression analysis of Patched 1 gene in the half-smooth tongue sole (Cynoglossus semilaevis). Dong Zhongdian,Qi Qian,Zhang Ning,Wen Haishen,Dong Zhongdian,Qi Qian,Zhang Ning,Shao Changwei,Zhang Liyan,Chen Songlin,Dong Zhongdian,Qi Qian,Zhang Ning,Shao Changwei,Zhang Liyan,Chen Songlin. 2016

[17]Genomic structure, polymorphism and expression analysis of the growth hormone (GH) gene in female and male Half-smooth tongue sole (Cynoglossus semilaevis). Ma, Qian,Liu, ShuFang,Zhuang, ZhiMeng,Lin, Lin,Sun, ZhongZhi,Liu, ChangLin,Ma, Hui,Tang, QiSheng,Ma, Qian,Lin, Lin,Su, YongQuan,Ma, Hui.

[18]Molecular characterization and expression profiles of LPXRFa at the brain pituitary-gonad axis of half-smooth tongue sole (Cynoglossus semilaevis) during ovarian maturation. Wang, Bin,Liu, Quan,Liu, Xuezhou,Xu, Yongjiang,Shi, Bao,Wang, Bin,Liu, Xuezhou,Xu, Yongjiang,Shi, Bao,Liu, Quan. 2018

[19]Effects of different dietary DHA: EPA ratios on gonadal steroidogenesis in the marine teleost, tongue sole (Cynoglossus semilaevis). Xu, Houguo,Cao, Lin,Wei, Yuliang,Zhang, Yuanqin,Liang, Mengqing,Liang, Mengqing. 2017

[20]Evaluation of reference genes for quantitative real-time PCR analysis of gene expression during early development processes of the tongue sole (Cynoglossus semilaevis). Ma Qian,Zhuang Zhimeng,Feng Wenrong,Liu Shufang,Tang Qisheng. 2015

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