Genetic verification of doubled haploid Japanese flounder, Paralichthys olivaceus by genotyping telomeric microsatellite loci
文献类型: 外文期刊
作者: Liu, Yongxin 1 ; Wang, Guixing 2 ; Liu, Yi 3 ; Hou, Jilun 3 ; Wang, Yufen 2 ; Si, Fei 2 ; Sun, Zhaohui 2 ; Zhang, Xiaoyan 2 ;
作者机构: 1.CAFS, Beijing 100141, Peoples R China
2.CAFS, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Peoples R China
3.NE Agr Univ, Harbin 150030, Heilongjiang, Peoples R China
关键词: Genetic map;Clone;Chromosome manipulation;Gynogenesis;Japanese flounder;Paralichthys olivaceus
期刊名称:AQUACULTURE ( 影响因子:4.242; 五年影响因子:4.723 )
ISSN: 0044-8486
年卷期: 2012 年 324 卷
页码:
收录情况: SCI
摘要: Mitotic gynogenetic diploid or doubled haploid Japanese flounder Paralichthys olivaceus were produced by activating eggs with UV irradiated sperm of red sea bream (Pagrus major), followed by hydrostatic pressure treatment to block the first mitotic division. To verify the doubled haploid, we measured the homozygosity of 21 maternally heterozygous microsatellite loci that are located in the telomeric region of chromosomes. These 21 loci have large marker-centromere distances of 43 to 50 cM estimated from four meiotic gynogenetic diploid lines, suggesting the location at telomeric regions of chromosomes. These 21 loci cover 16 out of the total 24 linkage groups of Japanese flounder. Microsatellite genotyping of these 21 markers in four mitotic gynogentic diploid lines shows that 75% (58 out of 77) individuals are homozygous at all 21 loci, indicating doubled haploids. The other 25% are heterozygous at 1 to 13 loci, probably representing spontaneously occurring meiotic gynogenetic diploids. These results demonstrate the high efficiency of generating doubled haploids in just one generation by mitotic gynogenesis. We have used these doubled haploids to successfully generate clonal lines via a second round of gynogenesis. (C) 2011 Elsevier B.V. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
Functional analysis of the ube3a response in Japanese flounder (Paralichthys olivaceus) to CSBV infection
作者:Su, Huaxing;Zhang, Yitong;He, Zhongwei;Yang, Yucong;Ren, Yuqin;Cao, Wei;Liu, Yufeng;Ren, Jiangong;Wang, Yufen;Wang, Guixing;Hou, Jilun;Su, Huaxing;Gong, Chunguang;Zhang, Yitong;He, Zhongwei;Yang, Yucong;Ren, Yuqin;Cao, Wei;Liu, Yufeng;Ren, Jiangong;Wang, Yufen;Wang, Guixing;Hou, Jilun
关键词:Japanese flounder; ube3a; Ubiquitination; Inflammatory response
-
Establishment of recombinant doubled haploid lines of Japanese flounder and evaluation of their reproductive capacity
作者:Zhang, Xiaoyan;Wang, Guixing;San, Lize;Zhang, Yitong;He, Zhongwei;Liu, Yufeng;Ren, Yuqin;Wang, Yufen;Hou, Jilun;Zhang, Xiaoyan;Wang, Guixing;San, Lize;Zhang, Yitong;He, Zhongwei;Liu, Yufeng;Ren, Yuqin;Wang, Yufen;Hou, Jilun
关键词:Recombinant doubled haploid; Japanese flounder; Reproductive traits; Gynogenesis; Targeted breeding
-
CoSFISH: a comprehensive reference database of COI and 18S rRNA barcodes for fish
作者:Wang, Yuanyuan;Yang, Yexin;Liu, Yi;Liu, Chao;Xu, Meng;Fang, Miao;Mu, Xidong;Yang, Yexin
关键词:
-
Identification and Characterization of microRNAs in Morphological Color Change of Polychromatic Midas Cichlids (Amphilophus citrinellus)
作者:Wu, Guoqiang;Mu, Xidong;Liu, Yi;Liu, Chao;Wang, Xuejie;Yang, Yexin;Song, Hongmei;Wu, Guoqiang
关键词:Midas cichlid; microRNAs; morphological color change; deep sequencing; target genes
-
Testing the applicability of the Modelling-Ongrowing Fish farms-Monitoring B (MOM-B) investigation system for assessing benthic habitat quality in the manila clam Ruditapes philippinarum aquaculture areas
作者:Liu, Yi;Wang, Xinmeng;Wu, Wenguang;Zhang, Jihong;Liu, Yi;Wang, Xinmeng;Wu, Wenguang;Zhang, Jihong
关键词:MOM-B system; Benthic habitat quality assessment; Manila clam
-
Characterization of caspase gene family in Sebastes schlegelii and their expression profiles under Aeromonas salmonicida and Vibrio anguillarum infection
作者:Yu, Haohui;Wang, Ningning;Liu, Xiantong;Xue, Ting;Li, Chao;Zhang, Xiaoyan;Yan, Xu;Yan, Xu
关键词:Caspase gene family; Sebastes schlegelii; Characterizations; Expression patterns
-
The first case of gynogenesis induced by cold- and heat-shock treatment in Thamnaconus modestus
作者:Zhang, Xiaoyan;San, Lize;Yang, Yucong;Tao, Yuehong;Ren, Jiangong;Liu, Yufeng;He, Zhongwei;Di, Jiashuo;Pei, Ziteng;Wang, Guixing;Hou, Jilun;Zhang, Xiaoyan;San, Lize;Yang, Yucong;Tao, Yuehong;Ren, Jiangong;Liu, Yufeng;He, Zhongwei;Di, Jiashuo;Pei, Ziteng;Wang, Guixing;Hou, Jilun;Di, Jiashuo;Pei, Ziteng
关键词:Thamnaconus modestus; Meiotic gynogenesis; Molecular markers; Homogeneity