Functional analysis of the ube3a response in Japanese flounder (Paralichthys olivaceus) to CSBV infection
文献类型： 外文期刊
作者： Su, Huaxing 1 ; Zhang, Yitong 1 ; He, Zhongwei 1 ; Yang, Yucong 1 ; Ren, Yuqin 1 ; Cao, Wei 1 ; Liu, Yufeng 1 ; Ren, Jiangong 1 ; Wang, Yufen 1 ; Wang, Guixing 1 ; Gong, Chunguang 2 ; Hou, Jilun 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Hebei Key Lab Bohai Sea Fish Germplasm Resources C, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Peoples R China
2.Hebei Agr Univ, Ocean Coll, Qinhuangdao 066009, Peoples R China
3.Chinese Acad Fishery Sci, Bohai Sea Fishery Res Ctr, Qinhuangdao 066100, Peoples R China
关键词： Japanese flounder; ube3a; Ubiquitination; Inflammatory response
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）
ISSN： 1050-4648
年卷期： 2024 年 148 卷
页码：
收录情况： SCI
摘要： Ube3a is a member of the E3 ubiquitin ligase HECTc family, and its role has been established in neurodevelopmental disorders. However, studies on its role in Japanese flounder are scarce. Thus, in this study, the ube3a of Japanese flounder was cloned, and its role in conferring resistance against Chinook salmon bafnivirus (CSBV) was analyzed. Japanese flounder ube3a encoded a protein containing 834 amino acids. Interestingly, its homology with the Atlantic halibut was determined to be 94%. In addition, there were differential expressions of ube3a in different tissues of Japanese flounder, with the highest expression level observed in the fin, followed by the gills and skin (P <= 0.05). Subcellular localization analysis revealed that Ube3a is a cytoplasmic protein. We established an in vitro CSBV infection model using Japanese flounder gill cell line (FG). After ube3a overexpression, the viral load was significantly lower than that of the control group (P <= 0.05). Contrastingly, after incubation of FG cells with an E3 ubiquitin ligase inhibitor, the viral load was significantly higher than in the control group (P <= 0.01). Then, the expression levels of nf-kappa b, traf3, and tnf-alpha after incubation with an E3 ubiquitin ligase inhibitor were examined. The results demonstrated that ube3a may exerted a significant antiviral effect in Japanese flounder via the ubiquitination pathway.
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