文献类型： 外文期刊

作者： Shah, Tahmina ¹ ; Malhi, Moolchand ¹ ; Kachiwal, Allah Bux ¹ ; Bhutto, Bachal ² ; Shah, Qurban Ali ³ ; Lei, Yan ⁴ ; Soomro, Saeed Ahmed ¹ ; Soomro, Jamila ¹ ; Kalhoro, Nazeer Hussain ⁵ ; Gui, Hongbing ⁶ ;

作者机构： 1.Sindh Agr Univ, Dept Vet Physiol & Biochem, Tandojam 70060, Pakistan

2.Sindh Agr Univ, Dept Vet Parasitol, Tandojam, Pakistan

3.Lasbela Univ Agr Water & Marine Sci, Dept Vet Pathol, Uthal, Balochistan, Pakistan

4.Henan Anim Husb Bur, Dairy Herd Improvement Ctr, Zhengzhou, Peoples R China

5.Sindh Inst Anim Hlth, Karachi, Pakistan

6.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing, Peoples R China

关键词： acute phase response; cytokines; interleukins; lipopolysaccharides; oxidative stress

期刊名称：FOOD SCIENCE & NUTRITION （ 影响因子：3.553； 五年影响因子：3.634 ）

ISSN： 2048-7177

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收录情况： SCI

摘要： We examined whether surplus dietary selenium (Se) supply could alleviate high concentrate (HC) diet-induced hepatic oxidative stress (OS) and inflammation. Eighteen young goats were distributed into three groups; were fed low (LC, concentrate: forage; 35: 65), high concentrate (HC, 65: 35), or Se-supplemented HC (HCSe, 65: 35 + 0.5 mg Se kg(-1) diet) diets for 10 weeks. Short chain fatty acids, OS markers and immunoinflammatory genes expressions were assessed through gas chromatograph, kits, and RT-qPCR, respectively. Compared with LC, HC diet increased (p < .05) colonic and serum lipopolysaccharide (LPS) levels and induced hepatic oxidative injury by increasing (p < .05) malondialdehyde (MDA) levels and decreasing (p < .05) activities of glutathione peroxidase, superoxide dismutase, and catalase. HC diet altered hepatic mRNA expressions of toll-like receptor-4 (TLR-4), cluster of differentiation-14 (CD-14), tumor necrosis factor-alpha (TNF-alpha), TNF receptor-associated factor-6 (TRAF-6), nuclear factor kappa B (NF-kappa B), interleukin-1 beta (IL-1 beta), IL-10, IL-13, LPS-binding protein (LBP), serum amyloid A (SAA), alpha-acid glycoprotein (AGP), and albumin (ALB). Conversely, extra-Se supply lowered LPS and attenuated antioxidant status and inflammation in liver. In conclusion, HC diet induced oxidative lesions and TLR-4 pathway-mediated inflammation, whereas supranutritional Se alleviated oxidative and inflammatory lesions through TLR-4 pathway regulation in goat liver.