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Stable cell-surface expression of Japanese flounder growth hormone in yeast Saccharomyces cerevisiae and growth-promoting effect on juvenile fish by oral administration

文献类型： 外文期刊

作者： Liu, Bin 2 ; Zang, Xiao-Nan 1 ; Liu, Xin-Fu 2 ; Zhang, Xue-Cheng 1 ; Lei, Ji-Lin 2 ;

作者机构： 1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China

2.Chinese Acad Fishery Sci, Qingdao Key Lab Marine Fish Breeding & Biotechnol, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China

关键词： Bioactivity;Cell surface display system;Paralichthys olivaceus;Recombinant growth hormone;Saccharomyces cerevisiae

期刊名称：FISHERIES SCIENCE （ 影响因子：1.617； 五年影响因子：1.531 ）

ISSN： 0919-9268

年卷期： 2012 年 78 卷 1 期

页码：

收录情况： SCI

摘要： A cost-effective method for producing and using recombinant growth hormone (GH) has been developed. A cell-surface protein display system was constructed to produce recombinant Japanese flounder growth hormone (r-JfGH) in yeast Saccharomyces cerevisiae and the biological activity of expressed r-JfGH was evaluated by oral delivery to juvenile Japanese flounder. The expression vector pYD-E2, containing the JfGH gene, S. cerevisiae GAL1 promoter, and the S. cerevisiae URA3 gene downstream and upstream sequences, was designed and constructed to integrate the GH gene into the yeast genome and secrete r-JfGH stably on the surface of the yeast cell. Expression of r-JfGH was confirmed by flow cytometry until the 50th continuous generation of transgenic yeast strain y-E2, and over 0.98 mu g r-JfGH per 1 mg total protein of y-E2 was estimated by an ELISA-RA assay. Growth of Japanese flounder was promoted significantly by the direct supplementation of freeze-dried transgenic yeast into the Japanese flounder juvenile feed at levels of 0.5 and 1%. The weight-specific growth rate and feed conversion ratio of the treated groups were 23.07 and 21.57% (0.5% feeding level) and 28.85 and 41.18% (1% feeding level), respectively, higher than that of the control group in a 7-week bioactivity study.

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