您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Inhibition of CYP450 1A and 3A by berberine in crucian carp Carassius auratus gibelio

文献类型： 外文期刊

作者： Zhou, Chang 1 ; Li, Xin-Cang 1 ; Fang, Wen-Hong 1 ; Yang, Xian-Le 3 ; Hu, Lin-Lin 1 ; Zhou, Shuai 1 ; Zhou, Jun-Fang 1 ;

作者机构： 1.Chinese Acad Fishery Sci, E China Sea Fisheries Res Inst, Key & Open Lab Marine & Estuarine Fisheries Resou, Minist Agr, Shanghai 200090, Peoples R China

2.Shanghai Ocean Univ, Sch Aquaculture & Life Sci, Shanghai 201306, Peoples R China

3.Shanghai Oc

关键词： Berberine;Crucian carp;CYP1A and 3A;Inhibition

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY （ 影响因子：3.228； 五年影响因子：3.289 ）

ISSN： 1532-0456

年卷期： 2011 年 154 卷 4 期

页码：

收录情况： SCI

摘要： Berberine has long been considered as an antibiotic candidate in aquaculture. However, studies regarding its effects on drug-metabolizing enzymes in fish are still limited. In the present study, the effects of berberine on cytochrome P4501A (CYP1A) and CYP3A in crucian carp were investigated. Injection of different concentrations of berberine (0, 5, 25, 50, and 100 mg/kg) inhibited the CYP1A mRNA expression, thereby inhibiting further the catalytic activity of CYP1A-related ethoxyresorufin-O-deethylase (EROD). Furthermore, both CYP1A expression and EROD activity were further inhibited with increasing berberine concentrations. In addition, the CYP3A expressions at both the mRNA and the protein levels were downregulated by higher berberine concentrations. The catalytic activity of CYP3A-related erythromycin N-demethylase (ERND) was also inhibited by berberine at a dose of no less than 25 mg/kg. Moreover, at the berberine concentration exceeding 25 mg/kg, the inhibition of CYP3A expression and ERND activity increased with increasing berberine concentrations. In vitro experiments were also performed. When berberine was pre-incubated with the crucian carp liver microsomes, it competitively inhibited the corresponding EROD activity with the IC50 of 11.7 mu M. However, the ERND activity was slightly inhibited by berberine with the IC50 of 206.4 mu M. These results suggest that, in crucian carp, berberine may be a potent inhibitor to CYP1A, whereas the CYP3A inhibition needs a higher concentration of berberine. (C) 2011 Elsevier Inc. All rights reserved.

  • 相关文献

[1]The Transcriptomes of the Crucian Carp Complex (Carassius auratus) Provide Insights into the Distinction between Unisexual Triploids and Sexual Diploids. Li, Chun-Yan,Kuang, You-Yi,Sun, Xiao-Wen,Li, Chun-Yan,Sun, Xiao-Wen,Li, Chun-Yan,Li, Jiong-Tang,Xu, Ru,Zhao, Zi-Xia,Hou, Guang-Yuan,Sun, Xiao-Wen,Li, Chun-Yan,Xu, Ru,Liang, Hong-Wei. 2014

[2]Isolation and characterization of microsatellite markers from Fangzheng silver crucian carp, Carassius auratus gibelio (Bloch), and cross-amplification in the closely related species crucian carp, Carassius auratus auratus (Linnaeus). Jia, Zhi-Ying,Sun, Xiao-Wen,Liang, Li-Qun,Lu, Cui-Yun,Lei, Qing-Quan. 2006

[3]Inhibiting sulfate reducing bacteria activity by denitrification in an anaerobic baffled reactor: influencing factors and mechanism analysis. Zhang, Zhaohan,Feng, Yujie,Ren, Nanqi,Zhang, Zhaohan,Liu, Guangmin,Chen, Zhongxi,Zhang, Zhaohan,Zhan, Peirong. 2014

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台