文献类型： 外文期刊
作者： Bian, Xueqiong 1 ; Ren, Xianyun 1 ; Jia, Shaoting 1 ; Gao, Tian 1 ; Wang, Junxia 5 ; Wang, Jiajia 1 ; Liu, Ping 1 ; Li, Jian 1 ; Li, Jitao 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Peoples R China
2.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
4.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
5.Rizhao Ocean & Fishery Res Inst, Marine Sci Res Ctr, Rizhao 276800, Peoples R China
关键词： Marsupenaeus japonicus; MjHSP90; cold stress; RNAi; apoptosis
期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）
ISSN：
年卷期： 2025 年 14 卷 8 期
页码：
收录情况： SCI
摘要： Molecular chaperones, especially heat shock proteins (HSPs) have vital functions in cells' responses to stress. Here, we cloned and sequenced the complete complementary DNA encoding HSP90 (MjHSP90) from the shrimp Marsupenaeus japonicus. The MjHSP90 cDNA comprised 3162 bp, including a 2172 bp coding region encoding a 724 amino acid-protein (predicted molecular mass = 83.12 kDa). Homology and phylogenetic analyses showed that MjHSP90 was highly conserved and most homologous to Litopenaeus vannamei HSP90. MjHSP90 is expressed in all tested tissues, with high expression in gill tissue and the hepatopancreas. Cold stress significantly upregulated MjHSP90 expression in the gill and hepatopancreas (p < 0.05). Following RNA interference knockdown of MjHSP90, the cold stress-related death rate of the shrimp increased significantly, accompanied by significantly upregulated expression of apoptosis-related genes Mjcaspase-3 and Mjbcl-2 (p < 0.05) and an increase in the number of apoptotic cells. The results indicated that MjHSP90 might play a pivotal role in the shrimp's immune response to cold stress.
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