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Comparative transcriptome analysis of brain and gonad reveals reproduction-related miRNAs in the giant prawn, Macrobrachium rosenbergii

文献类型： 外文期刊

作者： Xia, Jiao 1 ; Liu, Dong 1 ; Zhou, Wenzong 3 ; Yi, Shaokui 4 ; Wang, Xinhai 5 ; Li, Beilei 6 ; Jawad, Muhammad 1 ; Xu, Haijing 1 ; Gui, Lang 1 ; Li, Mingyou 1 ;

作者机构： 1.Shanghai Ocean Univ, Key Lab Integrated Rice fish Farming, Minist Agr & Rural Affairs, Shanghai, Peoples R China

2.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai, Peoples R China

3.Shanghai Acad Agr Sci, Inst Ecoenvironm Protect, Shanghai, Peoples R China

4.Huzhou Univ, Coll Life Sci, Huzhou, Zhejiang, Peoples R China

5.Jiangsu Acad Agr Sci, Suqian Inst Agr Sci, Suqian, Peoples R China

6.Huzhou Fengshengwan Aquat Seed Ind Co Ltd, Huzhou, Zhejiang, Peoples R China

关键词： macrobrachium rosenbergii; brain; gonad; RNA-seq; miRNA; reproduction; MiR-133; 5-HT1

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.772； 五年影响因子：4.933 ）

ISSN：

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Macrobrachium rosenbergii (M. rosenbergii), as a species of common prawn, is a delicacy that is consumed all over the world. By interacting with the target gene 3 & PRIME;-untranslated region (3'-UTR), microRNAs (miRNAs) regulate its expression and ultimately participate in the regulation of reproductive development. However, research focusing on miRNA regulation during gonadal development in M. rosenbergii received very little attention. To explore the association between miRNA and reproduction, we performed RNA sequencing (RNA-seq) on brain and gonad organs in male and female M. rosenbergii. A total of 494 miRNAs were obtained in RNA-seq, including 31 and 59 differentially expressed (DE) miRNAs in the brain and gonads, respectively. Furthermore, 9 DE miRNAs were randomly selected from the brain and gonads, and qRT-PCR was conducted to validate the results of RNA-seq. Interestingly, dpu-miR-133 was found to be substantially expressed in the male brain and testis but poorly expressed in the female brain, ovary, and other organs. Analysis of dpu-miR-133 by Targetscan and MiRanda predicted to target 5-HT1. Furthermore, the dual-luciferase reporter assay manifested that dpu-miR-133 can combine with 5-HT1. Overall, our research work provides basic data for further study on the miRNA-mediated regulation of brain, gonad, and reproductive development of study M. rosenbergii.

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